孕期蛋白质限制会改变后代牙胚中的RANKL/OPG系统。
Gestational protein restriction alters the RANKL/OPG system in the dental germ of offsprings.
作者信息
Calsa Bruno, Masiero Beatriz Calloni, Esquisatto Marcelo Augusto Marretto, Catisti Rosana, Santamaria Milton
机构信息
Graduate Program in Biomedical Sciences, University Center of Hermínio Ometto Foundation - FHO, Araras, SP, Brazil.
Graduate Program in Orthodontics, University Center of Hermínio Ometto Foundation, Araras, SP, Brazil.
出版信息
J Oral Biol Craniofac Res. 2020 Oct-Dec;10(4):743-746. doi: 10.1016/j.jobcr.2020.10.007. Epub 2020 Oct 13.
UNLABELLED
Evaluate the dentinogenesis in the offspring of rats submitted to gestational protein restriction (GPR).
DESIGN
The offspring were evaluated at the 21st day of gestation (21 dG). Assessments were made of morphological parameters and the RANKL/OPG system - bone tissue maturation markers - in the upper incisor tooth germ. Pregnant 10-week-old female Wistar rats were divided into normal protein (NP, 17% casein, n = 5) and low protein (LP, 6% casein, n = 5) diet groups. At 21 dG, the offspring maxillae were collected for histomorphometric and immunohistochemical analyses.
RESULTS
The LP group showed decreased thickness of the dentin and odontoblast cell layers on the tooth germ. GPR led to decreased OPG expression and increased RANKL expression in the incisor germ.
CONCLUSION
The results suggested that gestational protein restriction altered odontoblast RANKL/OPG expression and decreased dentin matrix deposition and thickness in tooth development.
未标记
评估孕期蛋白质限制（GPR）大鼠后代的牙本质形成。
设计
在妊娠第21天（21 dG）对后代进行评估。对上颌切牙牙胚的形态学参数以及RANKL/OPG系统（骨组织成熟标志物）进行评估。将10周龄的怀孕雌性Wistar大鼠分为正常蛋白质（NP，17%酪蛋白，n = 5）和低蛋白质（LP，6%酪蛋白，n = 5）饮食组。在21 dG时，收集后代的上颌骨进行组织形态计量学和免疫组织化学分析。
结果
LP组牙胚上的牙本质和成牙本质细胞层厚度降低。GPR导致切牙胚中OPG表达降低，RANKL表达增加。
结论
结果表明，孕期蛋白质限制改变了成牙本质细胞RANKL/OPG的表达，并降低了牙齿发育中牙本质基质的沉积和厚度。
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