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孕期蛋白质限制会增加成年雄性大鼠子代心脏中连接蛋白43的mRNA水平。

Gestational Protein Restriction Increases Cardiac Connexin 43 mRNA levels in male adult rat offspring.

作者信息

Rossini Kamila Fernanda, Oliveira Camila Andrea de, Rebelato Hércules Jonas, Esquisatto Marcelo Augusto Marreto, Catisti Rosana

机构信息

Programa de Pós Graduação em Ciências Biomédicas do Centro Universitário Hermínio Ometto FHO - UNIARARAS, Araras, SP - Brazil.

出版信息

Arq Bras Cardiol. 2017 Jul;109(1):63-70. doi: 10.5935/abc.20170081. Epub 2017 Jun 29.

DOI:10.5935/abc.20170081
PMID:28678925
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5524477/
Abstract

BACKGROUND

The dietary limitation during pregnancy influences the growth and development of the fetus and offspring and their health into adult life. The mechanisms underlying the adverse effects of gestational protein restriction (GPR) in the development of the offspring hearts are not well understood.

OBJECTIVES

The aim of this study was to evaluate the effects of GPR on cardiac structure in male rat offspring at day 60 after birth (d60).

METHODS

Pregnant Wistar rats were fed a normal-protein (NP, 17% casein) or low-protein (LP, 6% casein) diet. Blood pressure (BP) values from 60-day-old male offspring were measured by an indirect tail-cuff method using an electro sphygmomanometer. Hearts (d60) were collected for assessment of connexin 43 (Cx43) mRNA expression and morphological and morphometric analysis.

RESULTS

LP offspring showed no difference in body weight, although they were born lighter than NP offspring. BP levels were significantly higher in the LP group. We observed a significant increase in the area occupied by collagen fibers, a decrease in the number of cardiomyocytes by 104 µm2, and an increase in cardiomyocyte area associated with an increased Cx43 expression.

CONCLUSION

GPR changes myocardial levels of Cx43 mRNA in male young adult rats, suggesting that this mechanism aims to compensate the fibrotic process by the accumulation of collagen fibers in the heart interstitium.

摘要

背景

孕期饮食限制会影响胎儿和后代的生长发育以及他们成年后的健康。孕期蛋白质限制（GPR）对后代心脏发育产生不良影响的潜在机制尚不清楚。

目的

本研究旨在评估GPR对出生后60天（d60）雄性大鼠后代心脏结构的影响。

方法

给怀孕的Wistar大鼠喂食正常蛋白质（NP，17%酪蛋白）或低蛋白质（LP，6%酪蛋白）饮食。使用电子血压计通过间接尾袖法测量60日龄雄性后代的血压（BP）值。收集心脏（d60）用于评估连接蛋白43（Cx43）mRNA表达以及进行形态学和形态计量学分析。

结果

LP组后代出生时体重比NP组后代轻，但60日龄时体重无差异。LP组的血压水平显著更高。我们观察到胶原纤维所占面积显著增加，心肌细胞数量减少104 µm2，且心肌细胞面积增加，同时Cx43表达增加。

结论

GPR改变了雄性成年幼鼠心肌中Cx43 mRNA的水平，表明该机制旨在通过心脏间质中胶原纤维的积累来补偿纤维化过程。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/023d/5524477/a9dd54da545a/abc-109-01-0063-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/023d/5524477/fb85df3302e8/abc-109-01-0063-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/023d/5524477/fc46d40619b2/abc-109-01-0063-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/023d/5524477/44c7814039a2/abc-109-01-0063-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/023d/5524477/a9dd54da545a/abc-109-01-0063-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/023d/5524477/fb85df3302e8/abc-109-01-0063-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/023d/5524477/fc46d40619b2/abc-109-01-0063-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/023d/5524477/44c7814039a2/abc-109-01-0063-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/023d/5524477/a9dd54da545a/abc-109-01-0063-g04.jpg

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