Obi Linda U, Tekere Memory, Roopnarain Ashira, Sanko Tomasz, Maguvu Tawanda E, Bezuidenhout Cornelius C, Adeleke Rasheed A
Department of Environmental Sciences, University of South Africa, Johannesburg, South Africa.
Microbiology and Environmental Biotechnology Research Group, Institute for Soil, Climate and Water, Agricultural Research Council, Arcadia, 0083, Pretoria, South Africa.
Biotechnol Rep (Amst). 2020 Oct 9;28:e00542. doi: 10.1016/j.btre.2020.e00542. eCollection 2020 Dec.
Here, we report a high quality annotated draft genome of 39_H1, a Gram-negative facultative anaerobe that was isolated from an anaerobic digester. The strain exhibited hydrolytic/acidogenic properties by significantly improving methane production when used as a single isolate inoculum during anaerobic digestion of water hyacinth and cow dung. The total genome size of the isolate was 5,106,712 bp which corresponds to an N50 of 267,528 and G + C content of 59.7 %. Genome annotation with the NCBI Prokaryotic Genome Annotation Pipeline (PGAP) predicted a total of 4,908 genes of which 4,755 were protein coding genes; there were no plasmids detected. A number of genes associated with hydrolytic/acidogenic activities as well as other metabolic activities were identified and discussed.
在此，我们报告了39_H1的高质量注释基因组草图，39_H1是一种从厌氧消化池中分离出的革兰氏阴性兼性厌氧菌。该菌株在凤眼莲和牛粪的厌氧消化过程中作为单一分离接种物使用时，通过显著提高甲烷产量表现出水解/产酸特性。该分离株的基因组总大小为5,106,712 bp，N50为267,528，G+C含量为59.7%。使用NCBI原核生物基因组注释管道（PGAP）进行的基因组注释预测共有4,908个基因，其中4,755个为蛋白质编码基因；未检测到质粒。我们鉴定并讨论了一些与水解/产酸活性以及其他代谢活性相关的基因。
