Kalule John B, Tomusange Joseph, Namatovu Teddy
Department of Biotechnical and Diagnostic Sciences, College of Veterinary Medicine, Animal Resources Animal and Biosecurity, Kampala, Uganda.
Afr J Lab Med. 2020 Sep 30;9(1):864. doi: 10.4102/ajlm.v9i1.864. eCollection 2020.
Childhood brucellosis and malaria are co-endemic febrile illnesses in some sub-Saharan African countries. Malaria and brucellosis co-infection or brucellosis sole infections are often missed due to an over emphasis on malaria and the lack of appropriate diagnostic infrastructure. Brucellosis in dogs is usually overlooked and yet there is extensive contact between humans and their pets.
This study investigated brucellosis in children and dogs using a confirmatory serological testing series that screens for three sp.
Residual blood samples from malaria smear-negative febrile children were collected and tested for sp and malaria parasite. During the same period, residual blood samples presented to a veterinary microbiology laboratory in the same area were tested for brucellosis using the same approach.
A total of 105 human and 80 canine blood samples were tested for brucellosis antibodies. The seroprevalence of brucellosis was 22.86% (25/105) in children and 1.3% (1/80) in dogs using the Card, buffered acidified plate antigen, and standard plate agglutination tests but was 0% using the rivanol precipitation plate agglutination test.
Given that brucellosis can be caused by both smooth and rough colony strains, there is a need to modify the current serological surveillance strategy (targeted at only and other smooth colony strains) to figure out the relative contribution of rough colony strains ( and ). Since Uganda is endemic for brucellosis there is a need to modify the brucellosis surveillance strategy.
在一些撒哈拉以南非洲国家，儿童布鲁氏菌病和疟疾是共同流行的发热性疾病。由于过度重视疟疾以及缺乏适当的诊断基础设施，疟疾和布鲁氏菌病合并感染或单纯布鲁氏菌病感染常常被漏诊。犬类布鲁氏菌病通常被忽视，然而人类与其宠物之间有广泛接触。
本研究使用一系列确证性血清学检测方法对儿童和犬类的布鲁氏菌病进行调查，这些检测方法可筛查三种布鲁氏菌。
收集疟疾涂片阴性的发热儿童的剩余血样，检测布鲁氏菌和疟原虫。同一时期，将同一地区兽医微生物实验室收到的剩余血样采用相同方法检测布鲁氏菌病。
共对105份人类血样和80份犬类血样检测布鲁氏菌病抗体。使用平板凝集试验、缓冲酸化平板抗原试验和标准平板凝集试验，儿童布鲁氏菌病血清阳性率为22.86%（25/105），犬类为1.3%（1/80），但使用利凡诺沉淀平板凝集试验时血清阳性率为0%。
鉴于布鲁氏菌病可由光滑型和粗糙型菌落菌株引起，有必要修改当前的血清学监测策略（目前仅针对光滑型菌落菌株），以明确粗糙型菌落菌株（马尔他布鲁氏菌和流产布鲁氏菌）的相对贡献。由于乌干达是布鲁氏菌病的流行地区，有必要修改布鲁氏菌病监测策略。
