WHO WPRO Regional Polio Reference Laboratory and National Laboratory for Poliomyelitis, National Health Commission Key Laboratory for Biosafety, National Health Commission Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China.
Front Cell Infect Microbiol. 2020 Sep 25;10:475. doi: 10.3389/fcimb.2020.00475. eCollection 2020.
The subgenotype B5 of EV-A71 is a widely circulating subgenotype that frequently spreads across the globe. Several outbreaks have occurred in nations, such as Malaysia, Thailand, Vietnam, and Japan. Appearing first in Taiwan, China, the subgenotype has been frequently reported in mainland of China even though no outbreaks have been reported so far. The current study reconstructed the migration of the B5 subgenotype of EV-A71 in China via phylogeographical analysis. Furthermore, we investigated its population dynamics in order to draw more credible inferences. Following a dataset cleanup of B5 subgenotype of EV-A71, we detected earlier B5 subgenotypes of EV-A71 sequences that had been circulating in Malaysia and Singapore since the year 2000, which was before the 2003 outbreak that occurred in Sarawak. The Bayesian inference indicated that the most recent common ancestor of B5 subgenotype EV-A71 appeared in September, 1994 (1994.75). With respect to the overall prevalence, geographical reconstruction revealed that the B5 subgenotype EV-A71 originated singly from single-source cluster and subsequently developed several active lineages. Based on a large amount of data that was accumulated, we conclude that the appearance of the B5 subgenotype of EV-A71 in mainland of China was mainly due to multiple migrations from different origins.
肠道病毒 71 型（EV-A71）B5 亚基因型是一种广泛传播的亚基因型，经常在全球范围内传播。马来西亚、泰国、越南和日本等国家都曾发生过几次暴发。B5 亚基因型首先出现在中国台湾，尽管目前尚未报告暴发，但在中国大陆也经常被报道。本研究通过系统发育分析重建了中国 EV-A71 B5 亚基因型的传播情况。此外，我们还研究了其种群动态，以得出更可信的推论。对 EV-A71 B5 亚基因型进行数据集清理后，我们检测到了早在 2000 年就已经在马来西亚和新加坡流行的 B5 亚基因型 EV-A71 序列，这比 2003 年在砂拉越发生的疫情还要早。贝叶斯推断表明，B5 亚基因型 EV-A71 的最近共同祖先出现在 1994 年 9 月（1994.75）。就整体流行率而言，地理重建表明，B5 亚基因型 EV-A71 起源于单一来源的单源集群，随后发展出几个活跃的谱系。基于积累的大量数据，我们得出结论，中国大陆出现 EV-A71 B5 亚基因型主要是由于来自不同起源的多次迁移。
