WHO WPRO Regional Polio Reference Laboratory and National Health Commission Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China.
Xinjiang Uygur Autonomous Region Center for Disease Control and Prevention, Urumqi City, Xinjiang Uygur Autonomous Region, Beijing, People's Republic of China.
Emerg Microbes Infect. 2018 Nov 28;7(1):193. doi: 10.1038/s41426-018-0196-9.
Enterovirus B80 (EV-B80) is a newly identified serotype belonging to the enterovirus B species. To date, only two full-length genomic sequences of EV-B80 are available in GenBank, and few studies on EV-B80 have been conducted in China or worldwide. More information and research on EV-B80 is needed to assess its genetic characteristics, phylogenetic relationships, and association with enteroviral diseases. In this study, we report the phylogenetic characteristics of three Xinjiang EV-B80 strains and one Tibet EV-B80 strain in China. The full-length genomic sequences of four strains show 78.8-79% nucleotide identity and 94-94.2% amino acid identity with the prototype of EV-B80, indicating a tendency for evolution. Based on a maximum likelihood phylogenetic tree based on the entire VP1 region, three genotypes (A-C) were defined, revealing the possible origin of EV-B80 strains in the mainland of China. Recombination analysis revealed intraspecies recombinations in all four EV-B80 strains in nonstructural regions along with two recombination patterns. Due to the geographic factor, the coevolution of EV-B strains formed two different patterns of circulation. An antibody seroprevalence study against EV-B80 in two Xinjiang prefectures also showed that EV-B80 strains were widely prevalent in Xinjiang, China, compared to other studies on EV-B106 and EV-B89. All four EV-B80 strains are not temperature sensitive, showing a higher transmissibility in the population. In summary, this study reports the full-length genomic sequences of EV-B80 and provides valuable information on global EV-B80 molecular epidemiology.
肠道病毒 B80（EV-B80）是一种新鉴定的血清型，属于肠道病毒 B 种。迄今为止，GenBank 中仅提供了两个完整的 EV-B80 基因组序列，并且在中国或全球范围内对 EV-B80 的研究很少。需要更多关于 EV-B80 的信息和研究来评估其遗传特征、系统发育关系以及与肠道病毒病的关联。在本研究中，我们报告了中国新疆的三个 EV-B80 株和一个西藏 EV-B80 株的系统发育特征。四个菌株的全长基因组序列与 EV-B80 原型株的核苷酸同一性为 78.8-79%，氨基酸同一性为 94-94.2%，表明存在进化趋势。基于基于整个 VP1 区的最大似然系统发育树，定义了三个基因型（A-C），表明中国大陆 EV-B80 株的可能起源。重组分析显示，在四个 EV-B80 株的非结构区域均存在种内重组，并且存在两种重组模式。由于地理因素，EV-B 株的共同进化形成了两种不同的循环模式。对两个新疆地区针对 EV-B80 的抗体血清流行率研究也表明，与其他对 EV-B106 和 EV-B89 的研究相比，中国新疆的 EV-B80 株广泛流行。四个 EV-B80 株均不是温度敏感型，在人群中具有更高的传染性。总之，本研究报告了 EV-B80 的全长基因组序列，并提供了有关全球 EV-B80 分子流行病学的有价值信息。
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