Gruner S M
Princeton University, NJ 08544.
Science. 1987 Oct 16;238(4825):305-12. doi: 10.1126/science.3310232.
Instrumental and specimen considerations pertinent to performing time-resolved x-ray diffraction on biological materials are discussed. Existing synchrotron x-ray sources, in conjunction with integrating x-ray detectors, have made millisecond diffraction experiments feasible; exposure times several orders of magnitude shorter than this will be possible with synchrotron sources now on the drawing boards. Experience gained from time-resolved studies together with order-of-magnitude estimates of specimen requirements can be used to determine the instrumental capabilities needed for various time-resolved experiments. Existing instrumental capabilities and methods of dealing with time-resolved specimens are reviewed.
讨论了与对生物材料进行时间分辨X射线衍射相关的仪器和样本方面的考虑因素。现有的同步加速器X射线源，结合积分X射线探测器，已使毫秒级衍射实验成为可能；而目前正在设计中的同步加速器源将能够实现比这短几个数量级的曝光时间。从时间分辨研究中获得的经验以及对样本要求的数量级估计，可用于确定各种时间分辨实验所需的仪器能力。本文回顾了现有的仪器能力以及处理时间分辨样本的方法。
