新鲜及石蜡包埋材料中泌尿生殖系统癌细胞相对平均DNA含量的流式细胞术分析

Flow cytometric analysis of relative mean DNA content of urogenital cancer cells in fresh and paraffin-embedded materials.

作者信息

Nakamura K, Simon A L, Kasabian N G, Addonizio J C, Choudhury M, Nagamatsu G R, Rossi J A, Chiao J W

机构信息

Department of Urology, New York Medical College, Valhalla.

出版信息

Urology. 1987 Oct;30(4):333-6. doi: 10.1016/0090-4295(87)90295-0.

DOI:10.1016/0090-4295(87)90295-0

PMID:3310366

Abstract

The relative mean DNA content calculation was performed by flow cytometry on single cell suspensions prepared from fresh and paraffin-embedded specimens of 10 patients with surgically resected urogenital cancer. Samples were processed by a modified method of Hedley et al. including two hours of pepsinizing time, ribonuclease digestion, and propidium iodide staining. The mean DNA content which is a quantitative description of flow cytometric characteristics was significantly correlated between the fresh and paraffin-embedded materials (n = 10, r = 0.869, p less than 0.01). This method allows for the objective, retrospective analysis of DNA content in relation to diagnosis and prognosis of urogenital cancer.

摘要

通过流式细胞术对10例手术切除的泌尿生殖系统癌症患者的新鲜标本和石蜡包埋标本制备的单细胞悬液进行相对平均DNA含量计算。样本采用Hedley等人改良的方法处理，包括两小时的胃蛋白酶消化时间、核糖核酸酶消化和碘化丙啶染色。新鲜标本和石蜡包埋标本之间，作为流式细胞术特征定量描述的平均DNA含量显著相关（n = 10，r = 0.869，p < 0.01）。该方法允许对泌尿生殖系统癌症的诊断和预后相关的DNA含量进行客观的回顾性分析。