Stephenson R A, Gay H, Fair W R, Melamed M R
Cytometry. 1986 Jan;7(1):41-4. doi: 10.1002/cyto.990070106.
DNA content determinations were carried out by flow cytometry on nuclear suspensions prepared from the same paraffin-embedded tissue block for each of eight surgically resected human carcinomas at section thicknesses of 5,10,20,30,40,50, and 100 millimicrons. Flow cytometric DNA determinations were also obtained on fresh tissue specimens in four of the eight carcinomas. As section thickness decreased below 50 millimicrons, there was a progressive increase in the histogram baseline noise at low DNA values and a decrease in the relative peak height of aneuploid DNA. The former was attributed to an increase of nuclear fragments in thinner sections, and the latter to the greater probability of transection of the larger aneuploid cells within a specimen. Both artifacts were minimized at section thickness of 50 millimicrons or greater.
通过流式细胞术对从八个手术切除的人类癌组织的同一石蜡包埋组织块制备的核悬液进行DNA含量测定，切片厚度分别为5、10、20、30、40、50和100毫微米。还对八个癌组织中的四个新鲜组织标本进行了流式细胞术DNA测定。当切片厚度降至50毫微米以下时，低DNA值处的直方图基线噪声逐渐增加，非整倍体DNA的相对峰高降低。前者归因于较薄切片中核碎片的增加，后者归因于标本内较大非整倍体细胞横断的可能性增加。在50毫微米或更大的切片厚度下，这两种假象都被最小化。
