犊牛大肠杆菌败血症免疫中的交叉反应抗体。

Cross-reactive antibody in immunity to colisepticemia in calves.

作者信息

Wickstrom M L, Gay C C, Hodgson J L, Widders P R, Schaeffer D, Lee R, Corbeil L B

机构信息

College of Veterinary Medicine, Washington State University, Pullman 99164.

出版信息

Vet Microbiol. 1987 Mar;13(3):259-71. doi: 10.1016/0378-1135(87)90088-5.

DOI:10.1016/0378-1135(87)90088-5

PMID:3310376

Abstract

Cattle were immunized with a uridine diphosphate galactose epimerase deficient mutant of Escherichia coli to prepare antiserum cross-reactive with different serotypes of E. coli. Hypogammaglobulinemic calves were given bovine anti-J5 serum before oral challenge with virulent E. coli derived from a septicemic calf. Passively immunized calves had delayed and decreased bacteremia compared with calves given saline before challenge. Calves given antiserum also lived longer than control calves. A second experiment using ampicillin and antibody to treat colisepticemia also showed increased survival in anti-serum-treated calves. Decreased bacteremia was probably not due to the killing of the challenge strain by antibody and complement, as the strain was serum-resistant. However, anti-J5 serum did increase phagocytosis of the challenge strain of E. coli (JL9) by bovine neutrophils. Thus, partial protection by antiserum was probably due to increased clearance of bacteria as well as neutralization of endotoxin.

摘要

用大肠杆菌尿苷二磷酸半乳糖差向异构酶缺陷型突变体免疫牛，以制备与不同血清型大肠杆菌交叉反应的抗血清。在对源于败血症小牛的强毒大肠杆菌进行口服攻击之前，给低丙种球蛋白血症的小牛注射牛抗J5血清。与攻击前给予生理盐水的小牛相比，被动免疫的小牛菌血症出现延迟且程度减轻。给予抗血清的小牛也比对照小牛存活时间更长。另一项使用氨苄青霉素和抗体治疗大肠杆菌败血症的实验也表明，抗血清治疗的小牛存活率提高。菌血症减轻可能不是由于抗体和补体对攻击菌株的杀伤，因为该菌株具有血清抗性。然而，抗J5血清确实增加了牛中性粒细胞对大肠杆菌攻击菌株（JL9）的吞噬作用。因此，抗血清的部分保护作用可能是由于细菌清除增加以及内毒素中和。