Hubbard Peter, Velez Zélia
Centro de Ciências do Mar;
Centro de Ciências do Mar; Universidade do Algarve.
J Vis Exp. 2020 Oct 6(164). doi: 10.3791/60962.
Recent studies have shown that ocean acidification affects olfactory-driven behavior in fish. This may be due in part to a reduction in olfactory sensitivity in high PCO2/low pH water. To assess the effects of ocean acidification, or olfactory sensitivity in marine fish in general, we propose that extracellular multi-unit recording from the olfactory nerve is the method of choice. Although invasive, it is sensitive, robust, reproducible and independent of external salinity (unlike the electro-olfactogram [EOG], for example). Furthermore, it records a primary sensory input into the CNS, prior to any central processing. We show that this method can show a reduction in olfactory sensitivity that is both temporary and odorant-dependent, using a range of amino acids to construct concentration-response curves and calculate the thresholds of detection.
最近的研究表明，海洋酸化会影响鱼类由嗅觉驱动的行为。这可能部分归因于在高二氧化碳分压/低pH值的水中嗅觉敏感性降低。为了评估海洋酸化的影响，或者一般来说海洋鱼类的嗅觉敏感性，我们认为从嗅神经进行细胞外多单位记录是首选方法。尽管具有侵入性，但它灵敏、可靠、可重复且不受外部盐度影响（例如，与电嗅图[EOG]不同）。此外，它记录的是在任何中枢处理之前进入中枢神经系统的初级感觉输入。我们表明，使用一系列氨基酸构建浓度-反应曲线并计算检测阈值，这种方法可以显示出嗅觉敏感性的降低，这种降低既是暂时的，也是依赖于气味剂的。
