Shelby Emily A, Moss Jeanette B, Andreason Sharon A, Simmons Alvin M, Moore Allen J, Moore Patricia J
Department of Entomology, University of Georgia, Athens, GA 30602, USA.
U.S. Department of Agriculture, Agricultural Research Service, U.S. Vegetable laboratory, Charleston, SC 29414, USA.
Insects. 2020 Oct 22;11(11):723. doi: 10.3390/insects11110723.
The whitefly is a globally important pest that is difficult to control through insecticides, transgenic crops, and natural enemies. Post-transcriptional gene silencing through RNA interference (RNAi) has shown potential as a pest management strategy against . While genomic data and other resources are available to create highly effective customizable pest management strategies with RNAi, current applications do not capitalize on species-specific biology. This lack of specificity has the potential to have substantial ecological impacts. Here, we discuss both short- and long-term considerations for sustainable RNAi pest management strategies for , focusing on the need for species specificity incorporating both life history and population genetic considerations. We provide a conceptual framework for selecting sublethal target genes based on their involvement in physiological pathways, which has the greatest potential to ameliorate unintended negative consequences. We suggest that these considerations allow an integrated pest management approach, with fewer negative ecological impacts and reduced likelihood of the evolution of resistant populations.
粉虱是一种全球范围内重要的害虫，很难通过杀虫剂、转基因作物和天敌来控制。通过RNA干扰（RNAi）进行的转录后基因沉默已显示出作为一种防治害虫管理策略的潜力。虽然已有基因组数据和其他资源可用于利用RNAi创建高效的可定制害虫管理策略，但目前的应用并未利用物种特异性生物学特性。这种缺乏特异性有可能产生重大的生态影响。在此，我们讨论针对[具体害虫名称未给出]的可持续RNAi害虫管理策略的短期和长期考量，重点关注纳入生活史和种群遗传因素的物种特异性需求。我们提供了一个基于亚致死靶基因参与生理途径来选择它们的概念框架，这最有可能减轻意外的负面后果。我们建议这些考量能够实现综合害虫管理方法，减少负面生态影响，并降低抗性种群进化的可能性。
