Christiaens Olivier, Smagghe Guy
Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium.
Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium.
Curr Opin Insect Sci. 2014 Dec;6:15-21. doi: 10.1016/j.cois.2014.09.012. Epub 2014 Sep 28.
The post-transcriptional gene silencing mechanism RNA interference (RNAi) has potential as a crop protection strategy against important pest insects. Here we focus on Hemiptera pests, comprising some of the most devastating pest organisms as aphids, whiteflies, psyllids, bedbugs and kissing bugs. At first, a state-of-the-art overview is provided of the progress in RNAi in Hemiptera, as well as on the challenges when developing new RNAi-based pest control strategies against hemipteran pests, such as the delivery of dsRNA and degradation in the insect body. We also discuss the variability in RNAi efficiency as observed between species and experiments, and the factors potentially responsible for this phenomenon.
转录后基因沉默机制——RNA干扰（RNAi）具有作为一种针对重要害虫的作物保护策略的潜力。在这里，我们聚焦于半翅目害虫，其中包括一些最具破坏性的害虫生物，如蚜虫、粉虱、木虱、臭虫和锥蝽。首先，本文提供了半翅目RNAi研究进展的最新综述，以及在开发针对半翅目害虫的基于RNAi的新害虫控制策略时所面临的挑战，如双链RNA（dsRNA）的递送和在昆虫体内的降解。我们还讨论了在不同物种和实验中观察到的RNAi效率的变异性，以及可能导致这种现象的因素。
