Department of Entomology, MOA Key Lab of Pest Monitoring & Green Management, College of Plant Protection, China Agricultural University, Beijing, People's Republic China.
National Agricultural Technology Extension & Service Center, Beijing, People's Republic China.
Biotechniques. 2020 May;68(5):283-290. doi: 10.2144/btn-2019-0171. Epub 2020 Mar 23.
The application of RNAi promotes the development of novel approaches toward plant protection in a sustainable way. Genetically modified crops expressing dsRNA have been developed as commercial products with great potential in insect pest management. Alternatively, some nontransformative approaches, including foliar spray, irrigation and trunk injection, are favorable in actual utilization. In this review, we summarize the recent progress and successful cases of RNAi-based pest management strategy, explore essential implications and possibilities to improve RNAi efficiency by delivery of dsRNA through transformative and nontransformative approaches, and highlight the remaining challenges and important issues related to the application of this technology.
RNAi 的应用以可持续的方式促进了植物保护的新方法的发展。表达 dsRNA 的转基因作物已被开发为具有巨大潜力的商业产品，可用于昆虫害虫管理。或者，一些非转化方法，包括叶面喷施、灌溉和树干注射，在实际利用中是有利的。在这篇综述中，我们总结了基于 RNAi 的害虫管理策略的最新进展和成功案例，探讨了通过转化和非转化方法递送 dsRNA 来提高 RNAi 效率的重要意义和可能性，并强调了与该技术应用相关的剩余挑战和重要问题。
