Glander H J, Herrmann K, Haustein U F
Department of Dermatology, Karl-Marx-University, Leipzig/GDR.
Andrologia. 1987 Jul-Aug;19(4):456-9. doi: 10.1111/j.1439-0272.1987.tb02327.x.
Spermatozoa were prepared by swim up technique in the same way as for in vitro fertilization of human eggs and incubated with antihuman fibronectin antibodies in the immunofluorescence technique. Fibronectin mediates adhesive interactions between cells. 69 p.c. of spermatozoa with a normal shape and only 17 p.c. of them exhibiting a pathological shape showed a positive fibronectin-fluorescent band on the equatorial segment, called equatorial fibronectin band (EFB). The cryopreservation of semen did not influence the EFB. The significance of EFB for the sperm-oocyte-binding is discussed in connection with the sperm morphology and epididymal function.
精子采用上游法制备，方法与人类卵子体外受精相同，并在免疫荧光技术中与抗人纤连蛋白抗体一起孵育。纤连蛋白介导细胞间的黏附相互作用。69%形态正常的精子和仅17%形态异常的精子在赤道段显示出阳性的纤连蛋白荧光带，称为赤道纤连蛋白带（EFB）。精液的冷冻保存不影响EFB。结合精子形态和附睾功能讨论了EFB对精子-卵母细胞结合的意义。
