Department of Radiation Microbiology, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority, Cairo, Egypt.
Health Radiation Research Department, National Center for Radiation, Research and Technology, Egyptian Atomic Energy Authority, Cairo, Egypt.
Environ Toxicol. 2021 Apr;36(4):451-459. doi: 10.1002/tox.23050. Epub 2020 Oct 27.
d-Galactosamine (d-GalN) is a well-known toxin that causes many metabolic and morphological abnormalities resulting in advanced renal failure and liver damage. Occupational exposure to low-level ionizing radiation (<1 Gy) was shown to enhance cell protection via attenuating an established inflammatory process. The present study was therefore aimed to investigate the protective impact of Amphora coffaeiformis extract and low dose gamma radiation against d-GalN induced renal damage in rats. Forty-eight adult male Swiss albino rats were distributed equally into eight groups. The measurements included antioxidants activities (superoxide dismutase, catalase and glutathione peroxidase) as well as lipid peroxidation level in kidney tissue. Also, kidney function tests and inflammatory markers (tumor necrosis factor alpha and nuclear factor kappa-light-chain-enhancer of activated B cells) were measured. Additionally, relative quantification of kidney nuclear factor erythroid 2-related factor 2 (Nrf-2) gene was estimated. Histopathological examination was also performed in kidney tissue. The results revealed decreases in antioxidant activities and downregulation of Nrf-2 expression accompanied by increases in lipid peroxidation level, kidney function tests and inflammatory markers in d-GaIN group. The treatment with Amphora algal extract and low dose gamma radiation ameliorated the previous measurements which were harmony with histopathological findings. In conclusion, A coffaeiformis extract and low dose gamma radiation provided marked functional and histological effects in the treating acute renal damage induced by d-GalN in rats.
半乳糖胺（d-GalN）是一种众所周知的毒素，可导致多种代谢和形态异常，导致肾功能衰竭和肝损伤。职业性低水平电离辐射（<1Gy）暴露被证明可通过减弱已建立的炎症过程来增强细胞保护。因此，本研究旨在研究 Amphora coffaeiformis 提取物和低剂量伽马辐射对 d-GalN 诱导的大鼠肾损伤的保护作用。将 48 只成年雄性瑞士白化大鼠平均分配到 8 组。测量包括抗氧化剂活性（超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶）以及肾脏组织中的脂质过氧化水平。此外，还测量了肾功能试验和炎症标志物（肿瘤坏死因子-α和核因子 kappa-轻链增强子的 B 细胞）。还估计了肾脏核因子红细胞 2 相关因子 2（Nrf-2）基因的相对定量。还对肾脏组织进行了组织病理学检查。结果显示，d-GaIN 组的抗氧化活性降低，Nrf-2 表达下调，同时脂质过氧化水平、肾功能试验和炎症标志物升高。Amphora 藻类提取物和低剂量伽马辐射的治疗改善了以前的测量结果，这与组织病理学发现一致。总之，Amphora coffaeiformis 提取物和低剂量伽马辐射对 d-GalN 诱导的大鼠急性肾损伤具有显著的功能和组织学作用。
