Department of Ophthalmology, Bahçeşehir University Faculty of Medicine, İstanbul-Turkey.
Department of Orthopedics and Traumatology, T.C. Ministry of Health Adıyaman Kahta State Hospital, Adıyaman-Turkey.
Ulus Travma Acil Cerrahi Derg. 2020 Nov;26(6):853-858. doi: 10.14744/tjtes.2020.50384.
Algan Hemostatic Agent (AHA) is a multi-herbal extract containing a standardized amount of Achillea millefolium, Juglans regia, Lycopodium clavatum, Rubus caesius or Rubis fruciosus, Viscum album, and Vitis vinifera, each of which is effective in hemostasis. In this study, we aimed to investigate the effects of AHA on bleeding time in a rat tail hemorrhage model.
Forty-eight Sprague Dawley rats (5-7 weeks old, 180-210 g) were randomly and equally allocated to six groups as follows: heparin plus saline (heparinized control), heparin plus AHA-soaked sponge, heparin plus liquid form of AHA, saline (non-heparinized control), AHA-soaked sponge and liquid form of AHA. Heparin (640 IU/kg) was administered intraperitoneally three times a day for three days in heparinized groups. For the bleeding model, the tail of rats was transected. According to the study group, either saline- or AHA-soaked sponge or liquid form of AHA was applied over the hemorrhage area. In AHA- or saline-soaked sponge groups, once the bleeding time had started, it was checked every 10 seconds. If the bleeding did not stop after 40 seconds, it was accepted as a failure. In liquid AHA group, the duration of bleeding was measured using a chronometer and defined as the time (seconds) from wounding until the bleeding stopped.
Bleeding time in the heparinized and non-heparinized control groups was over 40 seconds. After applying the sponge form of AHA on the wound area, bleeding time was significantly shortened to less than 20 seconds in both heparinized and non-heparinized rats (p<0.001 for both). The liquid form of AHA stopped bleeding in 5.0±1.2 seconds and 8.0±1.3 seconds in heparinized and non-heparinized groups, respectively.
AHA is a highly effective topical hemostatic agent in a rat tail hemorrhage model, thus may provide for a unique clinically effective option for control of bleeding during surgical operations or other emergencies.
Algain 止血剂（AHA）是一种多草药提取物，含有标准化的西洋蓍草、胡桃、石松、黑穗醋栗或覆盆子、欧洲鹿蹄草和葡萄，每一种都具有止血作用。在这项研究中，我们旨在研究 AHA 对大鼠尾巴出血模型中出血时间的影响。
48 只 Sprague Dawley 大鼠（5-7 周龄，180-210 g）随机均分为六组：肝素加生理盐水（肝素化对照组）、肝素加 AHA 浸泡海绵、肝素加 AHA 液体制剂、生理盐水（非肝素化对照组）、AHA 浸泡海绵和 AHA 液体制剂。肝素化组每天腹腔内注射肝素 640 IU/kg，连续 3 天。对于出血模型，大鼠尾巴被切断。根据研究组，在出血区域应用生理盐水或 AHA 浸泡海绵或液体制剂。在 AHA 或生理盐水浸泡海绵组中，一旦出血开始，每 10 秒检查一次。如果 40 秒后出血未停止，则视为失败。在 AHA 液体制剂组中，使用秒表测量出血持续时间，并定义为从创伤到出血停止的时间（秒）。
肝素化和非肝素化对照组的出血时间超过 40 秒。在伤口区域应用 AHA 海绵形式后，肝素化和非肝素化大鼠的出血时间均显著缩短至 20 秒以下（两组均<0.001）。AHA 液体制剂在肝素化和非肝素化组中分别在 5.0±1.2 秒和 8.0±1.3 秒时停止出血。
AHA 是大鼠尾巴出血模型中一种非常有效的局部止血剂，因此可能为手术或其他紧急情况下控制出血提供一种独特的临床有效选择。
