Chatelus C, Carrier P, Saignes P, Libert M F, Berlier Y, Lespinat P A, Fauque G, Legall J
Section d'Enzymologie et Biochimie Bactérienne, Association pour la Recherche en Bioénergie Solaire, CEN Cadarache, Saint Paul Lez Durance, France.
Appl Environ Microbiol. 1987 Jul;53(7):1708-10. doi: 10.1128/aem.53.7.1708-1710.1987.
Batch cultures of Desulfovibrio vulgaris stored at 32 degrees C for 10 months have been found to retain 50% of the hydrogenase activity of a 1-day culture. The hydrogenase found in old cultures needs reducing conditions for its activation. Viable cell counts are negative after 6 months, showing that the hydrogenase activity does not depend on the presence of viable cells. These observations are of importance in the understanding of anaerobic biocorrosion of metals caused by depolarization phenomena.
已发现,在32摄氏度下储存10个月的普通脱硫弧菌分批培养物,其氢化酶活性保留了1天龄培养物的50%。在老龄培养物中发现的氢化酶需要还原条件来激活。6个月后活菌计数为阴性,表明氢化酶活性不依赖于活菌的存在。这些观察结果对于理解由去极化现象引起的金属厌氧生物腐蚀具有重要意义。