Department of Pharmacology, Maulana Azad University, Village Bujhawad, Teh-Luni, Jodhpur, Rajasthan, 342802, India.
Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
Cent Nerv Syst Agents Med Chem. 2020;20(3):186-193. doi: 10.2174/1871524920999201027125743.
Putranjiva roxburghii Wall is traditionally known to cure many pathological conditions including epilepsy.
The present study is aimed at determining bioactive compounds in ethanolic extract of Putranjiva roxburghii test extract (PRTE) seeds by GCMS analysis and to assess its antiepileptic potential using various experimental models of epilepsy.
The ethanolic extract of seeds of Putranjiva roxburghii was subjected to GC-MS analysis to detect the bioactive phytoconstituents. Acute oral toxicity of the extract was performed using OCED guideline 420. Pentylenetetrazol (PTZ) kindling model of epilepsy and Maximal electroshock epilepsy (MES) model of epilepsy were used to determine anti-epileptic potential.
The GC-MS analysis of the extract revealed the presence of 20 phytoconstituents. The major phytoconstituents included n-Propyl heptyl ether (25.25%), 5-Ethyl hydantoin (8%), octadec- 9-enoic acid (16.25%) and 1, 2-Benzene dicarboxylic acid (11.86%). The PRTE (50 mg/kg and 100 mg/kg) afforded a significant and dose-dependent protection against PTZ-induced kindling epilepsy and MES induced epilepsy (p<0.001 and p<0.01).
Based on the above findings, it is evident that Putranjiva roxburghii seeds contain biologically active compounds. It can also be concluded that the extract possesses anti-epileptic potential.
印度獐牙菜被传统医学用于治疗多种疾病，包括癫痫。
本研究旨在通过 GCMS 分析确定印度獐牙菜种子乙醇提取物（PRTE）中的生物活性化合物，并通过各种癫痫实验模型评估其抗癫痫潜力。
对印度獐牙菜种子的乙醇提取物进行 GC-MS 分析，以检测生物活性植物成分。采用 OCED 指南 420 进行提取物的急性口服毒性试验。戊四氮（PTZ）点燃癫痫模型和最大电休克癫痫（MES）模型用于确定抗癫痫潜力。
提取物的 GC-MS 分析显示存在 20 种植物成分。主要的植物成分包括正丙基庚基醚（25.25%）、5-乙基海因（8%）、十八烷-9-烯酸（16.25%）和 1,2-苯二甲酸（11.86%）。PRTE（50mg/kg 和 100mg/kg）对 PTZ 诱导的点燃性癫痫和 MES 诱导的癫痫具有显著的剂量依赖性保护作用（p<0.001 和 p<0.01）。
根据上述发现，印度獐牙菜种子含有具有生物活性的化合物。还可以得出结论，该提取物具有抗癫痫潜力。
