Drug Discovery and Development Division, Patanjali Research Institute, NH-58, Haridwar 249 405, Uttarakhand, India.
Department of Allied and Applied Sciences, University of Patanjali, Patanjali Yog Peeth, Rorkee-Haridwar Road, Haridwar 249 405, Uttarakhand, India.
Molecules. 2021 Feb 15;26(4):1020. doi: 10.3390/molecules26041020.
Putrajeevak ( Wall.; synonym (Wall.) Hurus) seeds have been used since ancient times in the treatment of infertility in the Ayurvedic system of medicine in India. In this study, the oil component of Putrajeevak seeds (PJSO) was extracted using the supercritical fluid extraction (SCFE) method using liquid CO and the constituents were analyzed using gas chromatography-flame ionized detectorand high-performance thin-layer chromatography. PJSO contained trace amounts of β-sitosterol with oleic and linoleic acids as the major fatty acid constituents. Male and female zebrafish were mutagenized with N-ethyl-N-nitrosourea (ENU) and fish that produced less than 20 viable embryos were selected for the study. SCFE oil extracts from the seeds were used in this study to reverse fertility impairment. The mutant fish were fed with PJSO for a period of 14 days and the rates of fertility, conception, and fecundity were determined with wild-type healthy fish as a breeding partner. Treatment with PJSO increased the ovarian follicle count as well as the number of mature eggs, while reducing the number of ovarian cysts. Sperm count as well as sperm motility were greatly enhanced in the ENU-mutagenized male zebrafish when treated with PJSO. The results obtained in this study demonstrate the effectiveness of seed oil in reversing impaired fertility in both male and female zebrafish models.
普特拉杰夫阿克（Wall.；同义词（Wall.）Hurus）种子自古以来就在印度的阿育吠陀医学系统中被用于治疗不孕不育。在这项研究中，使用超临界流体萃取（SCFE）法用液体 CO 提取普特拉杰夫阿克种子的油成分（PJSO），并使用气相色谱-火焰离子化检测器和高效薄层色谱法分析成分。PJSO 含有痕量的β-谷甾醇，油酸和亚油酸是主要的脂肪酸成分。雄性和雌性斑马鱼用 N-乙基-N-亚硝基脲（ENU）诱变，选择产生少于 20 个可存活胚胎的鱼进行研究。本研究中使用来自种子的 SCFE 油提取物来逆转生育能力受损。将突变鱼用 PJSO 喂养 14 天，并与野生型健康鱼作为繁殖伴侣确定生育力、受孕率和繁殖力。PJSO 处理增加了卵巢滤泡计数和成熟卵子数量，同时减少了卵巢囊肿数量。用 PJSO 处理后，ENU 诱变的雄性斑马鱼的精子计数和精子活力大大增强。本研究的结果表明，种子油在逆转雄性和雌性斑马鱼模型生育能力受损方面的有效性。
