上调的长链非编码 RNA LINC00858 促进胃癌细胞的增殖和迁移。

Up-regulated lncRNA LINC00858 facilitates proliferation and migration of gastric cancer cells.

机构信息

Department of General Surgery, The First People's Hospital of Huzhou, Zhejiang Province, China.

Department of General Surgery, Wuxing District People's Hospital, Huzhou City, Zhejiang Province, China.

出版信息

Pol J Pathol. 2020;71(3):236-243. doi: 10.5114/pjp.2020.99790.

DOI:10.5114/pjp.2020.99790

PMID:33112114

Abstract

Previous evidence has shown that the long intergenic non-protein coding RNA 858 (LINC00858) is an oncogene in non-small cell lung cancers. However, the role LINC00858 plays in gastric cancer (GC) is not clear. To illustrate the role LINC00858 plays in GC, the LINC00858 expression in GC and normal tissues was firstly detected. Then, the viability, proliferation and migration of GC BGC823 and MGC803 cells were assessed following LINC00858 knockdown by si-LINC00858 transfection. The results showed that LINC00858 had a high level of expressions in GC tissues as demonstrated by both online data and qRT-PCR assay. Also, the knockdown of LINC00858 reduced the proliferation and migration of BGC823 and MGC803 cells in vitro. Taken together, our data indicate that LINC00858 plays an oncogenic role in GC cells and might act as a potential therapeutic target for GC.

摘要

先前的证据表明，长链非编码 RNA 858（LINC00858）是非小细胞肺癌中的一种癌基因。然而，LINC00858 在胃癌（GC）中的作用尚不清楚。为了阐明 LINC00858 在 GC 中的作用，首先检测了 GC 和正常组织中的 LINC00858 表达。然后，通过 si-LINC00858 转染敲低 LINC00858，评估 GC BGC823 和 MGC803 细胞的活力、增殖和迁移。结果表明，在线数据和 qRT-PCR 检测均显示 LINC00858 在 GC 组织中高表达。此外，敲低 LINC00858 可减少 BGC823 和 MGC803 细胞在体外的增殖和迁移。总之，我们的数据表明，LINC00858 在 GC 细胞中发挥致癌作用，可能成为 GC 的潜在治疗靶点。