长链非编码 RNA LINC00858 的上调与胃癌的不良预后相关。

Up-regulation of long noncoding RNA LINC00858 is associated with poor prognosis in gastric cancer.

机构信息

Gastrointestinal Surgery, The first affiliated hospital of Shandong First medical university, Jinan, Shandong, China.

出版信息

J Gene Med. 2020 Jul;22(7):e3179. doi: 10.1002/jgm.3179. Epub 2020 Mar 17.

DOI:10.1002/jgm.3179

PMID:32119160

Abstract

BACKGROUND

The present study aimed to investigated the expression pattern of long noncoding RNA LINC00858 (LINC00858) in gastric cancer (GC) patients and its feasibility as a new prognostic biomarker.

METHODS

We examined LINC00858 expression in GC tissues and matched normal tissues from 189 patients using a quantitative reverse transcription-polymerase chain reaction. The correlations of LINC00858 levels in GC patients with clinicopathologic features were analyzed using a chi-squared test. The influence of LINC00858 on the overall survival rate of GC patients was precisely calculated using Kaplan-Meier methods (log rank tests). Multivariate Cox regression assays were carried out for the identification of the independent risk factors for GC.

RESULTS

We observed that LINC00858 was distinctly up-regulated in GC tissues compared to adjacent non-tumor specimens (p < 0.01). Higher expression of LINC00858 in GC was found to be associated with TNM stage (p = 0.003) and lymphatic metastasis (p = 0.007). Using Kaplan-Meier assays, we found that patients with high expression levels of LINC00858 had a distinctly poor overall survival and disease-free survival compared to those with low expression levels of LINC00858 (p = 0.0102). Multivariate analyses confirmed that LINC00858 (p < 0.05) was an independent prognosis factor for GC patients.

CONCLUSIONS

The data obtained in our study indicate that LINC00858 may be used as a novel prognostic indicator in GC patients.

摘要

背景

本研究旨在探讨长链非编码 RNA LINC00858（LINC00858）在胃癌（GC）患者中的表达模式及其作为新的预后生物标志物的可行性。

方法

我们使用定量逆转录聚合酶链反应（qRT-PCR）检测了 189 例 GC 患者肿瘤组织和配对正常组织中 LINC00858 的表达。采用卡方检验分析 LINC00858 水平与 GC 患者临床病理特征的相关性。Kaplan-Meier 法（log-rank 检验）精确计算 LINC00858 对 GC 患者总生存率的影响。采用多变量 Cox 回归分析鉴定 GC 的独立危险因素。

结果

与相邻非肿瘤标本相比，我们观察到 LINC00858 在 GC 组织中明显上调（p<0.01）。GC 中 LINC00858 的高表达与 TNM 分期（p=0.003）和淋巴转移（p=0.007）有关。Kaplan-Meier 分析显示，LINC00858 高表达的患者总生存率和无病生存率明显低于 LINC00858 低表达的患者（p=0.0102）。多因素分析证实 LINC00858（p<0.05）是 GC 患者的独立预后因素。