Department of Marine Biotechnology, National Kaohsiung University of Science and Technology, Kaohsiung City 811, Taiwan, ROC.
LIPM, Université de Toulouse, INRAE, CNRS, Castanet-Tolosan, France.
Int J Syst Evol Microbiol. 2020 Dec;70(12):6381-6389. doi: 10.1099/ijsem.0.004538. Epub 2020 Oct 27.
A novel Gram-negative, aerobic, non-motile, ovoid to rod-shaped bacterium, designated NBD-18, was isolated from a freshwater river in Taiwan. Optimal growth occurred at 30 °C, at pH 6 and in the absence of NaCl. The predominant fatty acids of strain NBD-18 were C, summed feature 3 (C 7 and/or C 6), C cyclo and summed feature 8 (C 7 and/or C 6). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidyldimethylethanolamine. The major polyamine was putrescine. The major isoprenoid quinone was Q-8. The genomic DNA G+C content of strain NBD-18 was 50.9 %. Strain NBD-18 was most closely related to LMG 29303 and HZ20 at a 16S rRNA gene sequence similarity of 97.7 %. 16S rRNA gene sequence similarity between LMG 29303 and HZ20 was 97.7 %. Phylogenetic analyses based on 16S rRNA gene sequences and an up-to-date bacterial core gene set indicated that strain NBD-18, LMG 29303 and HZ20 are affiliated with the same genus. Digital DNA-DNA hybridization, average nucleotide identity and average amino acid identity values among these three strains supported that they belong to the same genus and that strain NBD-18 represents a novel species. Thus, HZ20 should be reclassified as comb. nov. based on the rules for priority of publication and validation. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain NBD-18 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is NBD-18 (=BCRC 81197=LMG 31338).
一株新的革兰氏阴性、需氧、非运动、卵形到杆状的细菌，被命名为 NBD-18，从台湾的一条淡水河中分离得到。该菌最佳生长条件为 30°C、pH 值 6、无 NaCl。菌株 NBD-18 的主要脂肪酸为 C、总和特征 3（C 7 和/或 C 6）、C 环和总和特征 8（C 7 和/或 C 6）。主要极性脂为磷脂酰乙醇胺、磷脂酰甘油、双磷脂酰甘油和磷脂酰二甲乙醇胺。主要聚胺为腐胺。主要异戊二烯醌为 Q-8。菌株 NBD-18 的基因组 DNA G+C 含量为 50.9%。菌株 NBD-18 与 LMG 29303 和 HZ20 在 16S rRNA 基因序列相似性为 97.7%，与 LMG 29303 和 HZ20 的相似性为 97.7%。基于 16S rRNA 基因序列和最新的细菌核心基因集的系统发育分析表明，菌株 NBD-18、LMG 29303 和 HZ20 属于同一属。菌株 NBD-18、LMG 29303 和 HZ20 之间的数字 DNA-DNA 杂交、平均核苷酸同一性和平均氨基酸同一性值支持它们属于同一属，并且菌株 NBD-18 代表一个新种。因此，根据出版和验证的优先规则，HZ20 应重新归类为组合。nov.。基于基因型、化学分类和表型数据，菌株 NBD-18 代表属内的一个新种，建议使用新种名。模式菌株为 NBD-18（=BCRC 81197=LMG 31338）。
