College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, P. R. China.
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
J Mater Chem B. 2020 Dec 7;8(45):10407-10415. doi: 10.1039/d0tb01962c. Epub 2020 Oct 28.
Periodontitis is one of the most common inflammatory diseases that can eventually cause tooth loss in adults. For the successful regeneration of periodontal tissue, one of the most feasible ways is the development of functional guided tissue regeneration (GTR) membranes with improved osteogenic capability. Here, we fabricated electrospun silk fibroin (SF) nanofibrous membranes and designed a low-cost and eco-friendly strategy to modify the SF matrix via tannic acid (TA). In this process, the conformational transition of SF protein triggered by TA made a remarkable improvement in mechanical properties of the SF membranes. More importantly, TA could induce biomimetic mineralization and in situ growth of hydroxyapatite (HAp) on the surface of the SF nanofibrous matrix. Cell experiments demonstrated that TA-coated SF nanofibrous membranes after mineralization could facilitate the proliferation and osteo-differentiation of MC3T3 cells. Considering the effectivity and methodological simplicity, this TA-mediated modification is a promising method to prepare SF-based GTR membranes with better mechanical performance and osteogenic function.
牙周炎是最常见的炎症性疾病之一，最终可导致成年人牙齿脱落。为了实现牙周组织的成功再生，最可行的方法之一是开发具有增强成骨能力的功能性引导组织再生（GTR）膜。在这里，我们制备了静电纺丝丝素（SF）纳米纤维膜，并设计了一种低成本且环保的策略，通过单宁酸（TA）对 SF 基质进行修饰。在此过程中，TA 触发的 SF 蛋白构象转变使 SF 膜的机械性能得到显著改善。更重要的是，TA 可以诱导仿生矿化和原位生长羟基磷灰石（HAp）在 SF 纳米纤维基质的表面上。细胞实验表明，矿化后的 TA 涂层 SF 纳米纤维膜可以促进 MC3T3 细胞的增殖和成骨分化。考虑到有效性和方法的简单性，这种 TA 介导的修饰是一种很有前途的方法，可以制备具有更好机械性能和成骨功能的 SF 基 GTR 膜。
