Laboratory Genetic Metabolic Diseases, Amsterdam Gastroenterology & Metabolism, Amsterdam UMC, University of Amsterdam, the Netherlands.
Centre for Plant Sciences, School of Molecular and Cellular Biology, University of Leeds, UK.
FEBS Lett. 2021 Mar;595(6):763-772. doi: 10.1002/1873-3468.13974. Epub 2020 Nov 11.
ATP-binding cassette (ABC) subfamily D transporters are important for the uptake of fatty acids and other beta-oxidation substrates into peroxisomes. Genetic and biochemical evidence indicates that the transporters accept fatty acyl-coenzyme A that is cleaved during the transport cycle and then re-esterified in the peroxisomal lumen. However, it is not known whether free coenzyme A (CoA) is released inside or outside the peroxisome. Here we have used Saccharomyces cerevisiae and isolated peroxisomes to demonstrate that free CoA is released in the peroxisomal lumen. Thus, ABC subfamily D transporter provide an import pathway for free CoA that controls peroxisomal CoA homeostasis and tunes metabolism according to the cell's demands.
三磷酸腺苷结合盒（ABC）转运蛋白家族 D 亚家族在将脂肪酸和其他β-氧化底物摄取到过氧化物酶体中起着重要作用。遗传和生化证据表明，这些转运蛋白接受在运输循环中被切割的脂肪酸辅酶 A，然后在过氧化物酶体腔中重新酯化。然而，尚不清楚游离辅酶 A（CoA）是在过氧化物体内部还是外部释放。在这里，我们使用酿酒酵母和分离的过氧化物酶体证明游离 CoA 是在过氧化物酶体腔中释放的。因此，ABC 转运蛋白家族 D 亚家族为游离 CoA 提供了一种输入途径，控制着过氧化物酶体 CoA 的动态平衡，并根据细胞的需求调节代谢。
