Institute of General Microbiology, Christian-Albrechts University of Kiel, Germany.
FEBS Open Bio. 2020 Dec;10(12):2510-2531. doi: 10.1002/2211-5463.13016. Epub 2020 Nov 13.
Multiseriate and true-branching cyanobacteria are at the peak of prokaryotic morphological complexity. However, little is known about the mechanisms governing multiplanar cell division and morphogenesis. Here, we study the function of the prokaryotic cytoskeletal proteins, MreB and FtsZ in Fischerella muscicola PCC 7414 and Chlorogloeopsis fritschii PCC 6912. Vancomycin and HADA labeling revealed a mixed apical, septal, and lateral trichome growth mode in F. muscicola, whereas C. fritschii exhibits septal growth. In all morphotypes from both species, MreB forms either linear filaments or filamentous strings and can interact with FtsZ. Furthermore, multiplanar cell division in F. muscicola likely depends on FtsZ dosage. Our results lay the groundwork for future studies on cytoskeletal proteins in morphologically complex cyanobacteria.
多列和真分支蓝细菌处于原核形态复杂性的顶峰。然而，关于控制多平面细胞分裂和形态发生的机制知之甚少。在这里，我们研究了真核细胞骨架蛋白 MreB 和 FtsZ 在 Fischerella muscicola PCC 7414 和 Chlorogloeopsis fritschii PCC 6912 中的功能。万古霉素和 HADA 标记显示，F. muscicola 具有混合的顶端、隔膜和侧生毛状体生长模式，而 C. fritschii 则表现出隔膜生长。在来自两个物种的所有形态类型中，MreB 形成线性纤维或纤维状串，并且可以与 FtsZ 相互作用。此外，F. muscicola 中的多平面细胞分裂可能依赖于 FtsZ 剂量。我们的研究结果为未来研究形态复杂蓝细菌中的细胞骨架蛋白奠定了基础。
