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阿拉伯胶和单刺仙人掌提取物对HeLa细胞的抗增殖和诱导凋亡活性。

Anti-Proliferative and Apoptosis-Inducing Activity of Acacia Modesta and Opuntia Monocantha Extracts on HeLa Cells.

作者信息

Abid Farah, Saleem Muhammad, Muller Christian D, Asim Mulazim Hussain, Arshad Shumaila, Maqbool Tahir, Hadi Faheem

机构信息

Department of Pharmacy, Government College University of Faisalabad, Faisalabad, Pakistan.

Faculty of Pharmacy, University of Lahore, Defence Road, Lahore, Pakistan.

出版信息

Asian Pac J Cancer Prev. 2020 Oct 1;21(10):3125-3131. doi: 10.31557/APJCP.2020.21.10.3125.

DOI:10.31557/APJCP.2020.21.10.3125
PMID:33112576
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7798171/
Abstract

BACKGROUND

Cancer is one of the leading causes of death in the world. Numerous phytochemicals from plants have shown antineoplastic effects via programmed cell death (apoptosis). The aim of this study was to evaluate the effect of anti-proliferative and apoptosis-inducing activity of Acacia modesta and Opuntia monocantha against HeLa cells.

METHODS

To estimate anti-proliferative activity of the plants against HeLa cells, ethanol solvent was used for the extraction. For the evaluation of anti-proliferative effects, MTT assay was performed with 100, 200, and 400 µg/mL dose. The antioxidant assays including glutathione reductase (GSH), superoxide dismutase (SOD) and catalase were performed. Moreover, enzyme linked immunosorbent assay (ELISA) was performed. Furthermore, immunocytometry P53 and flow cytometry were also carried out to assess the apoptosis in HeLa cell.

RESULTS

MTT assay showed that the groups treated with Opuntia monocantha and Acacia modest have less level of toxicity as compared to untreated groups. Antioxidant assays confirmed that GSH, SPD and, catalase activities were quite decreased in treated groups as compared to untreated groups. Similarly, ELISA and apoptosis p53 have shown more pronounced apoptosis effect in treated groups as compared to untreated groups.

CONCLUSION

Based on above findings, treatment of HeLa cells with these plant extracts induced apoptosis, restricts proliferation, and enhances the anti-oxidative index in post treated cells.
.

摘要

背景

癌症是全球主要死因之一。许多植物中的植物化学物质已通过程序性细胞死亡（凋亡）显示出抗肿瘤作用。本研究的目的是评估阿拉伯金合欢和单刺仙人掌对人宫颈癌HeLa细胞的抗增殖和诱导凋亡活性。

方法

为评估植物对HeLa细胞的抗增殖活性，使用乙醇溶剂进行提取。为评估抗增殖作用，采用100、200和400μg/mL剂量进行MTT试验。进行了包括谷胱甘肽还原酶（GSH）、超氧化物歧化酶（SOD）和过氧化氢酶在内的抗氧化试验。此外，还进行了酶联免疫吸附测定（ELISA）。此外，还进行了免疫细胞化学检测P53和流式细胞术以评估HeLa细胞中的凋亡情况。

结果

MTT试验表明，与未处理组相比，用单刺仙人掌和阿拉伯金合欢处理的组毒性水平较低。抗氧化试验证实，与未处理组相比，处理组的GSH、SPD和过氧化氢酶活性明显降低。同样，与未处理组相比，ELISA和凋亡P53在处理组中显示出更明显的凋亡作用。

结论

基于上述发现，用这些植物提取物处理HeLa细胞可诱导凋亡、限制增殖并提高处理后细胞的抗氧化指数。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0489/7798171/58806315f6a2/APJCP-21-3125-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0489/7798171/923229f60942/APJCP-21-3125-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0489/7798171/40af83fca1b3/APJCP-21-3125-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0489/7798171/b185e8619b41/APJCP-21-3125-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0489/7798171/58806315f6a2/APJCP-21-3125-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0489/7798171/923229f60942/APJCP-21-3125-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0489/7798171/ac61aa0b7dc1/APJCP-21-3125-g002.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0489/7798171/58806315f6a2/APJCP-21-3125-g008.jpg

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