Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore, Lahore, Pakistan.
Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Science, Lahore, Pakistan.
Curr Pharm Biotechnol. 2019;20(15):1288-1308. doi: 10.2174/1389201020666190821144633.
Natural product with apoptotic activity could serve as a potential new source for anti-cancer medicine. Numerous phytochemicals from plants have shown to exert antineoplastic effects via programmed cell death (apoptosis). Cancer is one of the leading causes of death in prosperous countries. The subject study was intended to evaluate the anticancer properties of Kalonji extracts against cancer cell lines HeLa and HepG2 and normal cell lines BHK and VERO were used as normal controls.
MATERIALS & METHODS: For the evaluation of anti-proliferative effects, cell viability and cell death in all groups of cells were evaluated via MTT, crystal violet and trypan blue assays. For the evaluation of angiogenesis, Immunocytochemistry and ELISA of VEGF were done. Immunocytochemistry and ELISA of Annexin-V and p53 were performed for the estimation of apoptosis in all groups of cells. Furthermore, LDH assay, antioxidant enzymes activity (GSH, APOX, CAT and SOD) and RT-PCR with proliferative and apoptotic markers along with internal control were also performed. Cancer cells of both cell lines HepG2 and HeLa cells showed reduced viability, angiogenesis and proliferation with increased apoptosis when treated with Kalonji extracts. Whereas anti-oxidative enzymes show enhanced levels in treated cancer cells as compared to untreated ones.
It was observed that Kalonji extracts have the ability to induce apoptosis and improve the antioxidant status of HeLa and HepG2 cells. They can also inhibit the proliferation and angiogenesis in both these cancer cell lines.
具有凋亡活性的天然产物可以作为抗癌药物的潜在新来源。大量来自植物的植物化学物质已被证明通过程序性细胞死亡（细胞凋亡）发挥抗肿瘤作用。癌症是发达国家死亡的主要原因之一。本研究旨在评估 Kalonji 提取物对 HeLa 和 HepG2 癌细胞系的抗癌特性，并将 BHK 和 VERO 正常细胞系用作正常对照。
为了评估抗增殖作用，通过 MTT、结晶紫和台盼蓝测定法评估所有细胞组的细胞活力和细胞死亡。为了评估血管生成，进行了 VEGF 的免疫细胞化学和 ELISA。通过免疫细胞化学和 Annexin-V 和 p53 的 ELISA 评估所有细胞组的细胞凋亡。此外，还进行了 LDH 测定、抗氧化酶活性（GSH、APOX、CAT 和 SOD）以及 RT-PCR 与增殖和凋亡标志物以及内参。用 Kalonji 提取物处理 HepG2 和 HeLa 两种癌细胞系的癌细胞后，细胞活力、血管生成和增殖降低，凋亡增加。而与未处理的癌细胞相比，处理过的癌细胞中的抗氧化酶水平升高。
观察到 Kalonji 提取物具有诱导凋亡和改善 HeLa 和 HepG2 细胞抗氧化状态的能力。它们还可以抑制这两种癌细胞系的增殖和血管生成。
