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p21 ras家族癌基因产物在大肠正常黏膜及癌前和恶性肿瘤中的免疫细胞化学显示

Immunocytochemical demonstration of p21 ras family oncogene product in normal mucosa and in premalignant and malignant tumours of the colorectum.

作者信息

Kerr I B, Lee F D, Quintanilla M, Balmain A

出版信息

Br J Cancer. 1985 Nov;52(5):695-700. doi: 10.1038/bjc.1985.245.

DOI:10.1038/bjc.1985.245
PMID:3904802
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1977216/
Abstract

Study of the distribution of the p21 ras oncogene product as demonstrated by monoclonal antibody Y13-259 shows this protein to be apparently present in all epithelial populations of both premalignant and malignant tumours and throughout the normal foetal and adult epithelial crypt population in the colorectum. Metastatic tumour in liver shows a similar staining pattern which is less intense however than in the surrounding normal hepatocytes. Our results suggest that the presence of this protein is a widespread feature of normal cellular metabolism in certain cell types and is not restricted to those actively involved in cellular proliferation. It appears, furthermore, that neither cells at different stages of carcinogenesis nor those representing variants of a malignant phenotype can be identified using this particular antibody.

摘要

用单克隆抗体Y13 - 259对p21 ras癌基因产物分布的研究表明，该蛋白明显存在于癌前和恶性肿瘤的所有上皮细胞群体中，以及整个正常胎儿和成人结肠直肠上皮隐窝群体中。肝脏中的转移性肿瘤显示出类似的染色模式，不过其强度低于周围正常肝细胞。我们的结果表明，这种蛋白的存在是某些细胞类型正常细胞代谢的广泛特征，并不局限于那些积极参与细胞增殖的细胞。此外，似乎使用这种特定抗体无法识别处于癌变不同阶段的细胞或代表恶性表型变体的细胞。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b9d/1977216/807005b95aab/brjcancer00097-0019-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b9d/1977216/0852dfa0a214/brjcancer00097-0018-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b9d/1977216/0af43d58b7e2/brjcancer00097-0018-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b9d/1977216/807005b95aab/brjcancer00097-0019-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b9d/1977216/0852dfa0a214/brjcancer00097-0018-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b9d/1977216/0af43d58b7e2/brjcancer00097-0018-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b9d/1977216/807005b95aab/brjcancer00097-0019-a.jpg

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3
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本文引用的文献

1
Elevated expression of the human ras oncogene family in premalignant and malignant tumours of the colorectum.人类ras癌基因家族在结直肠癌癌前病变及恶性肿瘤中的表达升高。
Br J Cancer. 1984 Jun;49(6):681-8. doi: 10.1038/bjc.1984.108.
2
Activation of the mouse cellular Harvey-ras gene in chemically induced benign skin papillomas.化学诱导的良性皮肤乳头瘤中小鼠细胞哈维 - 拉斯基因的激活。
Nature. 1984;307(5952):658-60. doi: 10.1038/307658a0.
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Epidermal growth factor stimulates guanine nucleotide binding activity and phosphorylation of ras oncogene proteins.
用针对ras癌基因p21产物和碳水化合物决定簇抗原19-9的单克隆抗体对结肠直肠组织进行免疫组织化学染色。
J Clin Pathol. 1987 Feb;40(2):157-62. doi: 10.1136/jcp.40.2.157.
4
Elevated production of growth factor by human premalignant colon adenomas and a derived epithelial cell line.人癌前结肠腺瘤及衍生的上皮细胞系生长因子产生增加。
Br J Cancer. 1986 Nov;54(5):799-805. doi: 10.1038/bjc.1986.243.
5
Reactivity of monoclonal antibodies to oncoproteins with normal rat liver, carcinogen-induced tumours, and premalignant liver lesions.单克隆抗体与正常大鼠肝脏、致癌物诱导的肿瘤及癌前肝脏病变中癌蛋白的反应性。
Br J Cancer. 1988 Jan;57(1):48-53. doi: 10.1038/bjc.1988.7.
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Enhanced expression of ras gene products in psoriatic epidermis.ras基因产物在银屑病表皮中的表达增强。
Arch Dermatol Res. 1988;280(5):259-63. doi: 10.1007/BF00440597.
7
Immunohistochemical detection of ras oncogene p21 product in benign and malignant mammary tissue in man.人乳腺良恶性组织中ras癌基因p21产物的免疫组织化学检测
J Clin Pathol. 1986 Apr;39(4):428-34. doi: 10.1136/jcp.39.4.428.
8
Do all colorectal carcinomas arise in preexisting adenomas?所有结直肠癌都起源于先前存在的腺瘤吗？
World J Surg. 1989 Jan-Feb;13(1):45-51. doi: 10.1007/BF01671153.
9
Analysis of the c-Ha-ras-1 gene for deletion, mutation, amplification and expression in lymph node metastases of human head and neck carcinomas.分析人头颈癌淋巴结转移灶中c-Ha-ras-1基因的缺失、突变、扩增及表达情况。
Br J Cancer. 1990 Sep;62(3):398-404. doi: 10.1038/bjc.1990.306.
表皮生长因子刺激鸟嘌呤核苷酸结合活性并使ras癌基因蛋白磷酸化。
Nature. 1984;310(5973):147-50. doi: 10.1038/310147a0.
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Mol Cell Biol. 1984 Aug;4(8):1493-8. doi: 10.1128/mcb.4.8.1493-1498.1984.
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6
Monoclonal antibodies of predefined specificity detect activated ras gene expression in human mammary and colon carcinomas.具有预定义特异性的单克隆抗体可检测人乳腺癌和结肠癌中活化的ras基因表达。
Proc Natl Acad Sci U S A. 1984 Aug;81(16):5227-31. doi: 10.1073/pnas.81.16.5227.
7
The 1984 Walter Hubert lecture. Activation of transforming genes in neoplasms.1984年沃尔特·休伯特讲座。肿瘤中转化基因的激活。
Br J Cancer. 1984 Aug;50(2):137-42. doi: 10.1038/bjc.1984.155.
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Annu Rev Biochem. 1983;52:301-54. doi: 10.1146/annurev.bi.52.070183.001505.
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Transcription of c-onc genes c-rasKi and c-fms during mouse development.c-onc基因c-rasKi和c-fms在小鼠发育过程中的转录。
Mol Cell Biol. 1983 Jun;3(6):1062-9. doi: 10.1128/mcb.3.6.1062-1069.1983.