Yang Yan, Zhang Zhijie, Wan Menghui, Wang Zhihua, Zou Xueyan, Zhao Yanbao, Sun Lei
Engineering Research Center for Nanomaterials, Henan University, Kaifeng 475004, China.
Ministry of Education Key Laboratory of Advanced Civil Engineering Material, School of Materials Science and Engineering, and Institute for Advanced Study, Tongji University, Shanghai 201804, China.
Polymers (Basel). 2020 Oct 26;12(11):2486. doi: 10.3390/polym12112486.
Polyvinyl alcohol (PVA) electrospun nanofibers (NFs) are ideal carriers for loading silver nanoparticles (Ag NPs) serving as antibacterial materials. However, it is still a challenge to adjust the particles size, distribution, and loading density via a convenient and facile method in order to obtain tunable structure and antimicrobial activities. In this study, Ag NPs surface decorated PVA composite nanofibers (Ag/PVA CNFs) were fabricated by the solvothermal method in ethylene glycol, which plays the roles of both reductant and solvent. The morphology and structure of the as-fabricated Ag/PVA CNFs were characterized by scanning electron microscopy, transmission electron microscopy, selected area electron diffraction, X-ray diffraction, UV-visible spectroscopy, and Fourier transform infrared spectroscopy. Ag NPs had an average diameter of 30 nm, the narrowest size distribution and the highest loading density were successfully decorated on the surfaces of PVA NFs, at the AgNO concentration of 0.066 mol/L. The antibacterial properties were evaluated by the methods of absorption, turbidity, and growth curves. The as-fabricated Ag/PVA hybrid CNFs exhibit excellent antimicrobial activities with antibacterial rates over 98%, especially for the sample prepared with AgNO concentration of 0.066 mol/L. Meanwhile, the antibacterial effects are more significant in the Gram-positive bacteria of than the Gram-negative bacteria of , since PVA is more susceptive to . In summary, the most important contribution of this paper is the discovery that the particles size, distribution, and loading density of Ag NPs on PVA NFs can be easily controlled by adjusting AgNO concentrations, which has a significant impact on the antibacterial activities of Ag/PVA CNFs.
聚乙烯醇（PVA）电纺纳米纤维（NFs）是负载银纳米颗粒（Ag NPs）作为抗菌材料的理想载体。然而，通过便捷的方法调节颗粒大小、分布和负载密度以获得可调控的结构和抗菌活性仍然是一个挑战。在本研究中，采用溶剂热法在乙二醇中制备了Ag NPs表面修饰的PVA复合纳米纤维（Ag/PVA CNFs），乙二醇同时起到还原剂和溶剂的作用。通过扫描电子显微镜、透射电子显微镜、选区电子衍射、X射线衍射、紫外可见光谱和傅里叶变换红外光谱对所制备的Ag/PVA CNFs的形貌和结构进行了表征。在AgNO₃浓度为0.066 mol/L时，Ag NPs平均直径为30 nm，成功地在PVA NFs表面修饰了最窄的尺寸分布和最高的负载密度。通过吸收法、比浊法和生长曲线法评估了抗菌性能。所制备的Ag/PVA杂化CNFs表现出优异的抗菌活性，抗菌率超过98%，特别是对于AgNO₃浓度为0.066 mol/L制备的样品。同时，由于PVA对[此处原文缺失部分内容]更敏感，对革兰氏阳性菌的抗菌效果比对革兰氏阴性菌更显著。总之，本文最重要的贡献是发现通过调节AgNO₃浓度可以轻松控制PVA NFs上Ag NPs的颗粒大小、分布和负载密度，这对Ag/PVA CNFs的抗菌活性有显著影响。
