Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia (UPM), Serdang 43400, Selangor, Malaysia.
Ganoderma and Diseases Research of Oil Palm, Malaysian Oil Palm Board, Bandar Baru Bangi, Kajang 43000, Selangor, Malaysia.
Genes (Basel). 2020 Oct 26;11(11):1263. doi: 10.3390/genes11111263.
produces lignolytic enzymes that can degrade the lignin component of plant cell walls, causing basal stem rot to oil palms. Nitrogen sources may affect plant tolerance to root pathogens while hydrogen peroxide (HO), salicylic acid (SA) and jasmonic acid (JA) play important roles in plant defense against pathogens. In this study, we examined the expression of genes encoding manganese peroxidase (MnP) and laccase (Lac) in treated with different nitrogen sources (ammonium nitrate, ammonium sulphate, sodium nitrate and potassium nitrate), JA, SA and HO. Transcripts encoding MnP and Lac were cloned from . Of the three GbMnP genes, GbMnP_U6011 was up-regulated by all nitrogen sources examined and HO but was down-regulated by JA. The expression of GbMnP_U87 was only up-regulated by JA while was up-regulated by ammonium nitrate but suppressed by sodium nitrate and down-regulated by HO. Among the three genes examined, was up-regulated by ammonium nitrate, JA, SA and HO; was up-regulated by JA and HO while was up-regulated by SA but suppressed by ammonium sulphate, sodium nitrate, JA and HO. Differential expression of these genes may be required by their different functional roles in .
产生木质素降解酶,可降解植物细胞壁的木质素成分,导致油棕基部茎腐病。氮源可能会影响植物对根病原体的耐受性,而过氧化氢(HO)、水杨酸(SA)和茉莉酸(JA)在植物抵御病原体方面发挥着重要作用。在这项研究中,我们研究了不同氮源(硝酸铵、硫酸铵、硝酸钠和硝酸钾)、JA、SA 和 HO 处理后编码锰过氧化物酶(MnP)和漆酶(Lac)的基因在 中的表达。从 中克隆了编码 MnP 和 Lac 的转录本。在三个 GbMnP 基因中,GbMnP_U6011 被所有测试的氮源和 HO 上调,但被 JA 下调。GbMnP_U87 的表达仅被 JA 上调,而 仅被硝酸铵上调,但被硝酸钠和 HO 下调。在所研究的三个 基因中, 被硝酸铵、JA、SA 和 HO 上调; 被 JA 和 HO 上调,而 被 SA 上调,但被硫酸铵、硝酸钠、JA 和 HO 下调。这些基因的差异表达可能是由于它们在 中的不同功能作用所需要的。