College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China.
College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China.
Int J Biol Macromol. 2020 Dec 15;165(Pt B):2630-2639. doi: 10.1016/j.ijbiomac.2020.10.167. Epub 2020 Oct 26.
Okra, Abelmoschus esculentus (L.) Moench, an annual herbaceous plant, is widely distributed in tropical and subtropical regions. Water-soluble pectic hydrocolloids from okra stems (HOS) were extracted and purified using polydivinylbenzene HP-20 resins. The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02 contained galacturonic acid (34%), galactose (31%), rhamnose (21%), arabinose (4.2%), glucuronic acid (2.5%), xylose (1.2%), and other monosaccharides (6.1%) by weight. Its favorable rheological behaviors were evident on relatively higher concentrations (20, 25, and 30 mg/mL) and moderately lower pH levels (3 and 5) of HOS. The anti-fatigue experiments in vivo demonstrated that a high dose of HOS (450 mg/kg feed) prolonged the exhaustive swimming time of mice, significantly induced an increase in blood glucose and glycogen, and decreased lactic acid and serum urea nitrogen levels. HOS digestion in vivo was fairly conducive to the improvement of energy storage capacity and renal function for physically induced fatigue, compared with the conventional herbal supplement Panax quinquefolium. Accordingly, HOS exhibits potential for reutilization of okra stem waste.
黄秋葵，即 Abelmoschus esculentus（L.）Moench，是一种一年生草本植物，广泛分布于热带和亚热带地区。采用多乙烯基苯 HP-20 树脂提取和纯化黄秋葵茎中的水溶性果胶水溶胶（HOS）。纯化后的 HOS 的糖组成，重均分子量为 178.4±2.1 kDa，多分散指数为 1.02±0.02，其重量比含有半乳糖醛酸（34%）、半乳糖（31%）、鼠李糖（21%）、阿拉伯糖（4.2%）、葡萄糖醛酸（2.5%）、木糖（1.2%）和其他单糖（6.1%）。HOS 在相对较高浓度（20、25 和 30 mg/mL）和适度较低 pH 值（3 和 5）下具有良好的流变性能。体内抗疲劳实验表明，高剂量 HOS（450 mg/kg 饲料）延长了小鼠的力竭游泳时间，显著诱导血糖和糖原增加，降低了乳酸和血清尿素氮水平。与传统草药补充剂西洋参相比，HOS 对改善因体力活动引起的能量储存能力和肾功能更为有利。因此，HOS 具有再利用黄秋葵茎废物的潜力。
