Liu Suwen, Meng Fanna, Zhang Dong, Shi Donglin, Zhou Junyi, Guo Shuo, Chang Xuedong
College of Food Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao, China.
Hebei Yanshan Special Industrial Technology Research Institute, Qinhuangdao, China.
Front Nutr. 2022 Mar 9;9:853225. doi: 10.3389/fnut.2022.853225. eCollection 2022.
Exercise fatigue can exert deleterious effects on the body. This study evaluated the effects and mechanisms by which berry polyphenols extract (LCBP) improved the treadmill endurance of mice. Comparison was performed between the effects at 25°C and low temperatures (-5°C). Energy storage, product metabolism, and other biochemical indices were determined using vitamin C (VC) as a positive control. Co-immunoprecipitation was performed to detect the interaction between different proteins. Dietary supplementation with LCBP significantly prolonged the exhaustion time during treadmill exercise by 20.4% (25 °C) and 27.4% (-5 °C). LCBP significantly regulated the expression of antioxidant and inflammatory proteins, Bcl-2 /Bax apoptosis proteins, and the PKCα -NOx2 / Nox4 pathway proteins, and activated the expression of AMPK-PGC1α -NRF1-TFAM proteins in skeletal muscle mitochondria. The gene and protein expression of miRNA-133a/IGF-1/PI3K/Akt/mTOR in skeletal muscle cells was also activated. Molecular docking confirmed that the main components of LCBP such as cyanidin-3-glucoside, catechin, and chlorogenic acid, have strong binding affinity toward AMPKα. LCBP alleviates exercise fatigue in mice by reducing oxidative stress, inflammation, and apoptosis of skeletal muscle cells, enhances mitochondrial biosynthesis and cell proliferation, reduces fatigue, and enhances performance. These effects are also significant in a low-temperature environment (Graphical Abstract). Consequently, these results provide novel insights into the anti- fatigue roles of LCBP in exercise fatigue.
运动疲劳会对身体产生有害影响。本研究评估了浆果多酚提取物（LCBP）改善小鼠跑步机耐力的效果及机制。比较了其在25°C和低温（-5°C）下的作用效果。以维生素C（VC）作为阳性对照，测定能量储存、产物代谢及其他生化指标。进行免疫共沉淀以检测不同蛋白质之间的相互作用。膳食补充LCBP可显著延长跑步机运动中的疲劳时间，在25°C时延长20.4%，在-5°C时延长27.4%。LCBP显著调节抗氧化和炎症蛋白、Bcl-2/Bax凋亡蛋白以及PKCα-NOx2/Nox4信号通路蛋白的表达，并激活骨骼肌线粒体中AMPK-PGC1α-NRF1-TFAM蛋白的表达。骨骼肌细胞中miRNA-133a/IGF-1/PI3K/Akt/mTOR的基因和蛋白表达也被激活。分子对接证实，LCBP的主要成分如矢车菊素-3-葡萄糖苷、儿茶素和绿原酸对AMPKα具有很强的结合亲和力。LCBP通过减轻骨骼肌细胞的氧化应激、炎症和凋亡来缓解小鼠的运动疲劳，增强线粒体生物合成和细胞增殖，减轻疲劳并提高运动表现。在低温环境下这些作用也很显著（图摘要）。因此，这些结果为LCBP在运动疲劳中的抗疲劳作用提供了新的见解。
