Feng Chengbao, Wang Jingjing, Yang Xue, Zang Xuefang, Zhou Haikun, Zhang Erying, Li Haifei, Liu Bo, Chen Shumin, Wang Ying, Wang Peng, Gao Lixue, Liu Jinyan
Department of Medical Oncology, No. 2 Hospital of Baoding, Baoding City, People's Republic of China.
Baoding Children's Hospital, Baoding City, People's Republic of China.
Cancer Manag Res. 2020 Oct 13;12:10067-10075. doi: 10.2147/CMAR.S258565. eCollection 2020.
The purpose of this study was to prepare and characterize a lipid magnetic ball modified with KRAS antibodies on the surface and to isolate circulating tumor cells of colorectal cancer with mutations.
The microemulsion method was used to form lipid bilayers to encapsulate Fe3O4 nanoparticles with superparamagnetism to form lipid magnetic balls, and antibodies were formed on the surface to form immune lipid magnetic balls.
Compared with traditional EpCAM antibody-modified lipid magnetic balls, it can effectively improve the capture ability of colorectal cancer circulating tumor cells with mutation, the capture rate reaches 92.9%, and the capture results are consistent with clinical diagnosis and pathology.
Our results showed that antibody-modified lipid magnetic balls can be used in the diagnosis and treatment of colorectal cancer.
本研究旨在制备并表征表面修饰有KRAS抗体的脂质磁球，并分离出具有突变的结直肠癌循环肿瘤细胞。
采用微乳液法形成脂质双层以包裹具有超顺磁性的Fe3O4纳米颗粒，从而形成脂质磁球，并在表面形成抗体以形成免疫脂质磁球。
与传统的EpCAM抗体修饰的脂质磁球相比，它能有效提高对具有突变的结直肠癌循环肿瘤细胞的捕获能力，捕获率达到92.9%，且捕获结果与临床诊断及病理结果一致。
我们的结果表明，抗体修饰的脂质磁球可用于结直肠癌的诊断和治疗。
