MladenoviĆ Katarina G, GrujoviĆ Mirjana Ž, NikodijeviĆ Danijela D, ČomiĆ Ljiljana R
Department of Biology and Ecology, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, 34000 Kragujevac, Republic of Serbia.
Biosci Microbiota Food Health. 2020;39(4):227-233. doi: 10.12938/bmfh.2020-004. Epub 2020 Jul 4.
In this paper, we investigated the hydrophobicity, ability to adhere to solvents and the pig epithelium and co-aggregation of members of family Enterobacteriaceae and KGPMF 49. The bacteria used in this study were isolated from traditionally made autochthonous cheese from Southeastern Serbia (Sokobanja). The percentage of adhered bacteria was different in three solvents (chloroform, ethyl acetate and xylene). The highest percentage was detected in the presence of chloroform, and the lowest percentage was detected in the presence of xylene (chloroform < ethyl acetate < xylene). A different degree of co-aggregation of enterobacteria with KGPMF 49 was observed. KGPMF 8 demonstrated the highest percentage of co-aggregation with KGPMF49 (32.29%). KGPMF 13, KGPMF 9 and biogp 1 KGPMF 19 were found to have the ability to adhere to the pig epithelium, whereas KGPMF 22 showed no such ability. The ability to co-aggregate with other species and the ability to adhere to the pig epithelium are very important characteristics of the isolated bacteria.
在本文中,我们研究了肠杆菌科成员与KGPMF 49的疏水性、对溶剂和猪上皮细胞的黏附能力以及共聚集情况。本研究中使用的细菌是从塞尔维亚东南部(索科巴尼亚)传统制作的本地奶酪中分离出来的。在三种溶剂(氯仿、乙酸乙酯和二甲苯)中,黏附细菌的百分比有所不同。在氯仿存在的情况下检测到的百分比最高,在二甲苯存在的情况下检测到的百分比最低(氯仿<乙酸乙酯<二甲苯)。观察到肠杆菌与KGPMF 49有不同程度的共聚集。KGPMF 8与KGPMF49的共聚集百分比最高(32.29%)。发现KGPMF 13、KGPMF 9和生物型1的KGPMF 19具有黏附猪上皮细胞的能力,而KGPMF 22则没有这种能力。与其他物种共聚集的能力以及黏附猪上皮细胞的能力是分离出的细菌非常重要的特征。