Kos B, Susković J, Vuković S, Simpraga M, Frece J, Matosić S
Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia.
J Appl Microbiol. 2003;94(6):981-7. doi: 10.1046/j.1365-2672.2003.01915.x.
To investigate aggregation and adhesiveness of Lactobacillus acidophilus M92 to porcine ileal epithelial cells in vitro, and the influence of cell surface proteins on autoaggregation and adhesiveness of this strain.
Lactobacillus acidophilus M92 exhibits a strong autoaggregating phenotype and manifests a high degree of hydrophobicity determined by microbial adhesion to xylene. Aggregation and hydrophobicity were abolished upon exposure of the cells to pronase and pepsin. Sodium dodecyl sulphate polyacrylamide gel electrophoresis of cell surface proteins revealed the presence of potential surface layer (S-layer) proteins, approximated at 45 kDa, in L. acidophilus M92. The relationship between autoaggregation and adhesiveness to intestinal tissue was investigated by observing the adhesiveness of L. acidophilus M92 to porcine ileal epithelial cells. Removal of the S-layer proteins by extraction with 5 mol l-1 LiCl reduced autoaggregation and in vitro adhesion of this strain.
These results demonstrate that there is relationship between autoaggregation and adhesiveness ability of L. acidophilus M92, mediated by proteinaceous components on the cell surface.
This investigation has shown that L. acidophilus M92 has the ability to establish in the human gastrointestinal tract, which is an important determinant in the choice of probiotic strains.
体外研究嗜酸乳杆菌M92对猪回肠上皮细胞的聚集性和黏附性，以及细胞表面蛋白对该菌株自聚集和黏附性的影响。
嗜酸乳杆菌M92表现出强烈的自聚集表型，通过微生物对二甲苯的黏附测定显示出高度的疏水性。细胞经链霉蛋白酶和胃蛋白酶处理后，聚集性和疏水性消失。细胞表面蛋白的十二烷基硫酸钠聚丙烯酰胺凝胶电泳显示，嗜酸乳杆菌M92中存在潜在的表层（S层）蛋白，其分子量约为45 kDa。通过观察嗜酸乳杆菌M92对猪回肠上皮细胞的黏附性，研究了自聚集与对肠道组织黏附性之间的关系。用5 mol l-1 LiCl提取去除S层蛋白后，该菌株的自聚集和体外黏附性降低。
这些结果表明，嗜酸乳杆菌M92的自聚集与黏附能力之间存在关联，这种关联由细胞表面的蛋白质成分介导。
本研究表明嗜酸乳杆菌M92具有在人类胃肠道定殖的能力，这是选择益生菌菌株的一个重要决定因素。
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