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从韩国开菲尔中分离出的菌株在模拟胃肠道环境中的生存能力。

Survivability of Isolated From Korean Kefir in a Simulated Gastrointestinal Environment.

作者信息

Youn Hye-Young, Kim Dong-Hyeon, Kim Hyeon-Jin, Bae Dongryeoul, Song Kwang-Young, Kim Hyunsook, Seo Kun-Ho

机构信息

Center for One Health, College of Veterinary Medicine, Konkuk University, Seoul, South Korea.

Department of Food & Nutrition, College of Human Ecology, Hanyang University, Seoul, South Korea.

出版信息

Front Microbiol. 2022 Feb 24;13:842097. doi: 10.3389/fmicb.2022.842097. eCollection 2022.

Abstract

accounts for > 90% of the yeast population of kefir, and recently, its probiotic potential has been actively explored with a focus on its health benefits and safety. Herein, the survivability of five kefir-isolated strains (Km A1-A5) in a simulated gastrointestinal (GI) environment was evaluated and compared with those of commercial probiotic yeast, MYA-796. To further explore the potential to survive in the host GI tract, biochemical activities, hydrophobicity assay, biofilm formation, auto-aggregation analysis, and phenol tolerance of the strains were assessed. A4 exhibited the best survivability among all tested strains, including the clinically proven probiotic yeast strain MYA-796 ( = 0.014) in the artificial GI tract ranging from pH 2.0 to 7.5. In addition, the five strains and MYA-796 displayed different assimilation of lactose, xylitol, D-sorbitol, and DL-lactate, indicating that metabolized a wide range of substances and, thus, might be more feasible to nourish themselves in the host GI tract for survival. strains showed a greater hydrophobicity of cell surface, abilities to biofilm formation and auto-aggregation, and phenol tolerance than MYA-796, suggesting greater potential for survival in the host GI tract.

摘要

占开菲尔酵母菌群的90%以上,最近,人们积极探索其益生菌潜力,重点关注其健康益处和安全性。在此,评估了五株从开菲尔中分离的菌株(Km A1 - A5)在模拟胃肠道(GI)环境中的存活能力,并与商业益生菌酵母MYA - 796进行了比较。为了进一步探索在宿主胃肠道中存活的潜力,评估了这些菌株的生化活性、疏水性测定、生物膜形成、自聚集分析和对苯酚的耐受性。在从pH 2.0到7.5的人工胃肠道中,A4在所有测试菌株中表现出最佳的存活能力,包括临床验证的益生菌酵母菌株MYA - 796(P = 0.014)。此外,这五株菌株和MYA - 796对乳糖、木糖醇、D - 山梨醇和DL - 乳酸的同化作用不同,表明它们能代谢多种物质,因此在宿主胃肠道中自我滋养以生存可能更可行。这些菌株比MYA - 796表现出更大的细胞表面疏水性、生物膜形成和自聚集能力以及对苯酚的耐受性,表明在宿主胃肠道中具有更大的存活潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a58/8908258/65ff70229856/fmicb-13-842097-g001.jpg

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