Ngalani Ornella Jt, Marbou Wiliane Jt, Mbaveng Armelle Tsafack, Kuete Victor
Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon.
Osong Public Health Res Perspect. 2020 Oct;11(5):319-326. doi: 10.24171/j.phrp.2020.11.5.08.
This study aimed to investigate the immunological and bacterial profiles in pregnant women of Bafang-Cameroon.
Stool and midstream urine were cultured using specific culture media. The disk diffusion method was used for the antimicrobial susceptibility test. T-cell lymphocyte counts (CD3, CD4 and CD8), white blood cell counts, sensitive C-reactive protein, and interleukin-6, were measured by flow cytometry, optical detection, and the enzyme-linked immunosorbent assay solid phase direct sandwich method.
Out of 700 participants, 71.43% were pregnant, and 28.57% were non-pregnant women. The mean age was 29.40 ± 8.27 and 27.41 ± 6.55 years in non-pregnant and pregnant women, respectively. CD4 T-cells were not significantly lower in pregnant women compared with non-pregnant women. There were 43.65% and 56.35% bacteria isolates obtained from urine and stool samples, respectively. Bacteria were mostly isolated in patients with a CD4 T-cell count between 461 and 806 cells/μL. Isolates of and showed 100% resistance in non-pregnant women, however all isolated bacteria were shown to be multidrug resistant in pregnant women. . (24.3%) and (21.51%) showed an increase in multidrug resistant phenotypes in pregnant women.
This study demonstrated that routine bacteriological analysis during pregnancy is necessary for their follow-up care.
本研究旨在调查喀麦隆巴方地区孕妇的免疫学和细菌学特征。
使用特定培养基对粪便和中段尿进行培养。采用纸片扩散法进行药敏试验。通过流式细胞术、光学检测和酶联免疫吸附测定固相直接夹心法测定T细胞淋巴细胞计数（CD3、CD4和CD8）、白细胞计数、超敏C反应蛋白和白细胞介素-6。
在700名参与者中，71.43%为孕妇，28.57%为非孕妇。非孕妇和孕妇的平均年龄分别为29.40±8.27岁和27.41±6.55岁。与非孕妇相比，孕妇的CD4 T细胞无显著降低。分别从尿液和粪便样本中分离出43.65%和56.35%的细菌。细菌大多在CD4 T细胞计数为461至806个细胞/微升的患者中分离出来。在非孕妇中，[具体细菌名称1]和[具体细菌名称2]的分离株显示100%耐药，然而在孕妇中，所有分离出的细菌均显示为多重耐药。[具体细菌名称3]（24.3%）和[具体细菌名称4]（21.51%）在孕妇中显示多重耐药表型增加。
本研究表明，孕期进行常规细菌学分析对其后续护理是必要的。
