Research Unit of Microbiology and Antimicrobial Substances, Department of Biochemistry, University of Dschang, P.O. Box 67, Dschang, Cameroon.
Biomed Res Int. 2021 Oct 4;2021:4303902. doi: 10.1155/2021/4303902. eCollection 2021.
The wound healing process is essential to reform the damaged tissue and prevent its invasion by pathogens. The present study aims at evaluating the antibacterial and therapeutic properties of the L. (Solanaceae) extract against infected wound in a rat model with its mechanisms of antibacterial action. The fruit extract was prepared by maceration in methanol. The broth microdilution method was used to investigate the antibacterial activity of the methanol extract of fruits. The therapeutic effect of the extract gel was performed on an excision wound infected with using a rat model. The total phenol, flavonoid, and tannin contents as well as the antibacterial mechanisms of action of the extract were determined using spectrophotometric methods. The fruit extract showed antibacterial properties which can be linked to its total phenolic, flavonoid, and tannin contents. The antibacterial activity is due to the inhibition of the biofilm formation, ATPases/H proton pump, and dehydrogenase activity as well as the alteration of the bacterial cell membrane through the leakage of nucleic acids, reducing sugars and proteins. The extract gel showed a significant ( < 0.05) increase in the percentage of wound closure and eradicated at the infection site. The extract gel was nonirritating to the skin and slightly irritating to the eyes and should be used with caution. Overall, the findings of the present study support the traditional use of the studied plant in the treatment of wounds and infectious diseases associated with the tested bacteria.
伤口愈合过程对于修复受损组织和防止病原体入侵至关重要。本研究旨在评估 （茄科）果实提取物对感染创面的抗菌和治疗特性，并研究其抗菌作用机制。采用甲醇浸渍法制备果实提取物。采用肉汤微量稀释法研究 果实甲醇提取物的抗菌活性。采用大鼠模型，在感染 的切创创面中进行提取物凝胶的治疗效果。采用分光光度法测定提取物的总酚、类黄酮和单宁含量以及抗菌作用机制。 果实提取物具有抗菌特性，这与其总酚、类黄酮和单宁含量有关。抗菌活性是由于抑制生物膜形成、ATP 酶/H 质子泵和脱氢酶活性以及通过核酸、还原糖和蛋白质的渗漏改变细菌细胞膜。提取物凝胶显著（<0.05）增加了创面闭合百分比，并在感染部位消除了 。提取物凝胶对皮肤无刺激性，对眼睛有轻微刺激性，应谨慎使用。总的来说，本研究的结果支持了传统上使用研究植物治疗与测试细菌相关的伤口和感染性疾病的做法。
