Zühlke H, Lendeckel U, Neumer C, Brandt R
Institute of Biochemistry, Ernst-Moritz-Arndt-University, Greifswald/ GDR.
Exp Clin Endocrinol. 1987 Aug;89(3):319-24. doi: 10.1055/s-0029-1210657.
A polymorphic locus flanking the 5'-end of the insulin gene was studied by means of the recombinant DNA technique in 62 diabetic and nondiabetic subjects. The small allele predominantly was found with the following frequencies: 28 insulin-dependent diabetics 0.89; 6 probands of a family with one type I diabetic member 0.92; 10 patients with a non-insulin-dependent diabetes mellitus 0.55; 18 control probands (normal glucose tolerance) 0.64. --Because of the relatively high number of all probands with the small allele we assume that the presence of this allele on both chromosomes increases the susceptibility but is not sufficient for the onset of an insulin-dependent diabetes mellitus. By a correlation of the polymorphic region of the insulin gene with other DNA regions (in particular with the major histocompatibility complex) the susceptibility may be further increased. Therefore, the HLA-DR gene polymorphism of type I diabetes is of interest.
采用重组DNA技术，在62名糖尿病患者和非糖尿病患者中研究了胰岛素基因5'-端侧翼的一个多态位点。发现小等位基因主要具有以下频率：28名胰岛素依赖型糖尿病患者为0.89；一个有一名I型糖尿病成员的家族中的6名先证者为0.92；10名非胰岛素依赖型糖尿病患者为0.55；18名对照先证者（糖耐量正常）为0.64。——由于所有携带小等位基因的先证者数量相对较多，我们认为两条染色体上都存在该等位基因会增加易感性，但不足以引发胰岛素依赖型糖尿病。通过将胰岛素基因的多态区域与其他DNA区域（特别是与主要组织相容性复合体）进行关联，易感性可能会进一步增加。因此，I型糖尿病的HLA-DR基因多态性备受关注。
