Hormozgan University of Medical Sciences Hormozgan Health Institute Molecular Medicine Research Center Bandar Abbas Iran Molecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Hormozgan University of Medical Sciences Faculty of Medicine Department of Biochemistry Bandar Abbas Iran Department of Biochemistry, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Braz J Cardiovasc Surg. 2020 Oct 1;35(5):697-705. doi: 10.21470/1678-9741-2019-0377.
To investigate the association between interleukin-35 (IL-35) levels and single nucleotide polymorphisms (rs3761548, rs3761547) of the FoxP3 gene in coronary artery bypass grafting (CABG) patients.
We conducted a prospective study including 140 patients, who were scheduled for elective isolated on-pump CABG with cardiopulmonary bypass (CPB) from January 2017 to September 2018 in the Jorjani heart center. Blood samples were collected before and 12 hours after the operation. Serum levels of IL-35 were measured by enzyme-linked immunosorbent assay and the pattern of genetic variations was assessed using single specific primer-polymerase chain reaction.
The serum concentrations of IL-35 after surgery were significantly higher than pre-surgery levels (18.4±8.3 vs. 9.89±3.2, respectively, P=0.002). There was no significant association between genotype frequencies of rs3761548 and rs3761547 and elevated IL-35 levels (P>0.05). There were significant associations between IL-35 levels and preoperative variables, including age (r=-0.34, P=0.047) and body mass index (r=-0.41, P=0.045), and intraoperative variables, including CPB time (r=0.4, P=0.02) and mean arterial pressure (r=-0.38, P=0.046), in carriers of the rs3761548 AA genotype.
Serum IL-35 concentrations were significantly increased in CPB patients, which may contribute to the post-CPB compensatory anti-inflammatory response syndrome. IL-35 increased levels were not influenced by FoxP3 promoter polymorphisms (rs3761548, rs3761547).
探讨白细胞介素-35（IL-35）水平与 FoxP3 基因单核苷酸多态性（rs3761548、rs3761547）在冠状动脉旁路移植术（CABG）患者中的关系。
我们进行了一项前瞻性研究，纳入了 140 名患者，这些患者于 2017 年 1 月至 2018 年 9 月在 Jorjani 心脏中心接受了择期单纯体外循环下 CABG。在手术前和手术后 12 小时采集血液样本。通过酶联免疫吸附试验测量血清中 IL-35 的浓度，并用单特异性引物-聚合酶链反应评估遗传变异模式。
手术后的血清 IL-35 浓度明显高于术前水平（18.4±8.3 比 9.89±3.2，P=0.002）。rs3761548 和 rs3761547 基因型频率与升高的 IL-35 水平之间无显著相关性（P>0.05）。IL-35 水平与术前变量（包括年龄 r=-0.34，P=0.047 和体重指数 r=-0.41，P=0.045）和术中变量（包括 CPB 时间 r=0.4，P=0.02 和平均动脉压 r=-0.38，P=0.046）显著相关，在 rs3761548 AA 基因型携带者中。
CPB 患者的血清 IL-35 浓度显著升高，这可能有助于 CPB 后代偿性抗炎反应综合征。FoxP3 启动子多态性（rs3761548、rs3761547）对 IL-35 水平的升高没有影响。
