黑麦菜花：A431 表皮癌细胞的细胞毒性评价及非靶向 LC-HR-MS/MS 代谢物分析。
Verbascum nigrum: Cytotoxicity Evaluation in A431 Epidermoid Carcinoma Cells and Untargeted LC-HR-MS/MS Metabolite Profiling.
机构信息
Department of Pharmacognosy, Grigore T. Popa University of Medicine and Pharmacy, 700115, Iasi, Romania.
Phytopharmacy and Natural Products Research Group, Institute of Chemistry and Biotechnology, Zurich University of Applied Sciences (ZHAW), 8820, Wädenswil, Switzerland.
出版信息
Chem Biodivers. 2020 Dec;17(12):e2000644. doi: 10.1002/cbdv.202000644. Epub 2020 Dec 1.
The crude methanolic extract obtained from Verbascum nigrum aerial parts (VNE) and its six fractions (VNF1-VNF6) were initially screened regarding their effects on the viability of immortalized HaCaT keratinocytes and A431 epidermoid carcinoma cells (MTT assay, 24 h). None of the tested samples affected the viability of HaCaT cells in a concentration range of 25-150 μg/mL. VNE and VNF4 exhibited significant cytotoxic effects in A431 cells, with IC values of 81.92 and 12.27 μg/mL, respectively; the selectivity index was higher than 10 for VNF4. The untargeted LC/HR-MS/MS metabolite profiling led to the tentative annotation of a total number of 23 compounds. Of these, VNE comprised mainly iridoid glycosides (harpagoside, laterioside, acylated aucubin derivatives), whereas VNF4 showed a high abundance of triterpene saponin glycosides (ilwensisaponins A and C, songarosaponins A and B), constituents known for their selective cytotoxic potential.
从黑麦草地上部分（VNE）及其六个馏分（VNF1-VNF6）获得的粗甲醇提取物最初根据其对永生化 HaCaT 角质形成细胞和 A431 表皮癌细胞活力的影响进行了筛选（MTT 测定法，24 h）。在 25-150μg/mL 的浓度范围内，测试的样品均未影响 HaCaT 细胞的活力。VNE 和 VNF4 在 A431 细胞中表现出显著的细胞毒性作用，IC 值分别为 81.92 和 12.27μg/mL；VNF4 的选择性指数高于 10。非靶向 LC/HR-MS/MS 代谢产物分析导致了 23 种化合物的暂定注释。其中，VNE 主要包含环烯醚萜苷（獐牙菜苦苷、獐牙菜苷、酰化梓醇衍生物），而 VNF4 则显示出大量三萜皂苷糖苷（伊万里皂苷 A 和 C、松栌皂苷 A 和 B）的高丰度，这些成分因其选择性细胞毒性潜力而闻名。
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