Yagi Sakina, Nilofar Nilofar, Uba Abdullahi Ibrahim, Caprioli Giovanni, Mustafa Ahmed M, Angeloni Simone, Koyuncu Ismail, Seker Fatma, Polat Rıdvan, Supti Sumaiya Jahan, Tasnim Faria, Al Dhaheri Yusra, Zengin Gokhan, Eid Ali H
Department of Botany, Faculty of Science, University of Khartoum, Khartoum, Sudan.
Université de Lorraine, INRAE, LAE, Nancy, France.
Front Pharmacol. 2024 Jan 30;15:1333865. doi: 10.3389/fphar.2024.1333865. eCollection 2024.
The present study was designed to evaluate the chemical composition, antioxidant, enzyme inhibition and cytotoxic properties of different extracts from aerial parts of (family Scrophulariaceae), a plant that is native to Lebanon, Syria and Turkey. Six extracts, namely, hexane, dichloromethane (DCM), ethyl acetate (EtOAc), ethanol (EtOH), 70% EtOH, and water (aqueous) were prepared by maceration. The EtOH extract was predominated by the presence of rutin (4280.20 μg g) and -coumaric acid (3044.01 μg g) while the highest accumulation of kaempferol-3-glucoside (1537.38 μg g), caffeic acid (130.13 μg g) and 4-hydroxy benzoic acid (465.93 μg g) was recorded in the 70% EtOH, aqueous, and EtOAc extracts, respectively. The EtOH (46.86 mg TE/g) and 70% EtOH (46.33 mg TE/g) extracts displayed the highest DPPH radical scavenging result. Both these extracts, along with the aqueous one, exerted the highest ABTS radical scavenging result (73.03-73.56 mg TE/g). The EtOH and 70% EtOH extracts revealed the most potent anti-AChE (2.66 and 2.64 mg GALAE/g) and anti-glucosidase (1.07 and 1.09 mmol ACAE/g) activities. The aqueous extract was the most efficacious in inhibiting the proliferation of prostate cancer (DU-145) cells with an IC of 8.71 μg/mL and a Selectivity Index of 3.7. In conclusion, this study appraised the use of aerial parts as a potential therapeutic source for future development of phytopharmaceuticals that target specific oxidative stress-linked diseases including diabetes, cancer, cardiovascular disease, and Alzheimer's disease among others.
本研究旨在评估玄参科一种原产于黎巴嫩、叙利亚和土耳其的植物地上部分不同提取物的化学成分、抗氧化、酶抑制和细胞毒性特性。通过浸渍法制备了六种提取物，即己烷、二氯甲烷（DCM）、乙酸乙酯（EtOAc）、乙醇（EtOH）、70%乙醇和水提取物。乙醇提取物中芦丁（4280.20μg/g）和对香豆酸（3044.01μg/g）含量较高，而山奈酚-3-葡萄糖苷（1537.38μg/g）、咖啡酸（130.13μg/g）和4-羟基苯甲酸（465.93μg/g）的最高积累量分别出现在70%乙醇、水和乙酸乙酯提取物中。乙醇提取物（46.86mg TE/g）和70%乙醇提取物（46.33mg TE/g）表现出最高的DPPH自由基清除效果。这两种提取物以及水提取物表现出最高的ABTS自由基清除效果（73.03 - 73.56mg TE/g）。乙醇提取物和70%乙醇提取物显示出最强的抗乙酰胆碱酯酶（2.66和2.64mg GALAE/g）和抗葡萄糖苷酶（1.07和1.09mmol ACAE/g）活性。水提取物对前列腺癌（DU - 145）细胞增殖的抑制效果最为显著，IC50为8.71μg/mL，选择性指数为3.7。总之，本研究评估了该植物地上部分作为潜在治疗来源的用途，可为未来开发针对包括糖尿病、癌症、心血管疾病和阿尔茨海默病等特定氧化应激相关疾病的植物药提供依据。
