School of Environmental and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu 212003, China.
School of Environmental and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu 212003, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Feb 15;247:119074. doi: 10.1016/j.saa.2020.119074. Epub 2020 Oct 15.
A novel purine Schiff base fluorescent probe (WYW), (E)-4-methyl-2-((2-(9-(naphthalen-1-yl)-8-(thiophen-2-yl)-9H-purin-6-yl)hydrazono)methyl)phenol, was designed and prepared as an excellent reversible fluorescent chemosensor for monitoring Al. The fluorogenic "turn-on" sensor WYW exhibited high selectivity towards Al over other coexistent metal ions, accompanying with an obvious visual color change in DMSO/HO (9/1, v/v, pH = 7.4) media. The enhancement fluorescence of WYW could be attributed to the inhibition of PET and ESIPT process induced by Al. Notably, the WYW-Al complex exhibited a fluorescence "turn-off" response towards F with exceptional selectivity via the displacement approach. The detection limit of WYW for Al was calculated to be as low as 82 nM. The formation of complex WYW-Al (1:1 stoichiometry) was confirmed by Job's methods and further verified by density functional theory (DFT) calculations. Furthermore, the probe WYW with low cytotoxicity and excellent membrane-permeable property has also been successfully applied for detecting low concertation Al in living HeLa cells.
一种新型嘌呤席夫碱荧光探针（WYW），（E）-4-甲基-2-((2-(9-(萘-1-基)-8-(噻吩-2-基)-9H-嘌呤-6-基)亚肼基)甲基)苯酚，被设计并制备为用于监测 Al 的优异的可逆荧光化学传感器。生色“开启”传感器 WYW 对 Al 具有高度选择性，相对于其他共存的金属离子，在 DMSO/HO（9/1，v/v，pH = 7.4）介质中伴随明显的视觉颜色变化。WYW 的增强荧光归因于 Al 诱导的 PET 和 ESIPT 过程的抑制。值得注意的是，WYW-Al 配合物通过置换方法对 F 表现出异常选择性的荧光“关闭”响应。WYW 对 Al 的检测限计算低至 82 nM。通过 Job 的方法和进一步通过密度泛函理论（DFT）计算验证了配合物 WYW-Al（1:1 化学计量比）的形成。此外，具有低细胞毒性和优异膜渗透性的探针 WYW 已成功应用于检测活 HeLa 细胞中的低浓度 Al。
