Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan, Shanxi 030001, China.
Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, Shandong Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Feb 15;247:119038. doi: 10.1016/j.saa.2020.119038. Epub 2020 Oct 5.
Aptamers are short single-stranded RNA or DNA molecules that can recognize a series of targets with high affinity and specificity. Known as "chemical antibodies", aptamers have many unique merits, including ease of chemical synthesis, high chemical stability, low molecular weight, lack of immunogenicity, and ease of modification and manipulation compared to their protein counterparts. Using aptamers as the recognition groups, fluorescent aptasensors provide exciting opportunities for sensitive detection and quantification of analytes. Herein, we give an overview on the recent development of aptamer-based fluorescent sensors for the detection of cancer biomarkers. Based on various nanostructured sensor designs, we extended our discussions on sensitivity, specificity and the potential applications of aptamer-based fluorescent sensors in early diagnosis, treatment and prognosis of cancers.
适体是能够高亲和力和特异性识别一系列靶标的短单链 RNA 或 DNA 分子。被称为“化学抗体”，适体具有许多独特的优点，包括易于化学合成、化学稳定性高、分子量低、缺乏免疫原性，以及与蛋白质相比易于修饰和操作。使用适体作为识别基团，荧光适体传感器为分析物的灵敏检测和定量提供了令人兴奋的机会。本文综述了基于适体的荧光传感器用于检测癌症生物标志物的最新进展。基于各种纳米结构的传感器设计，我们扩展了关于基于适体的荧光传感器在癌症的早期诊断、治疗和预后中的灵敏度、特异性和潜在应用的讨论。
