肺支原体菌株间的蛋白质变异性。

Watson H L, Davidson M K, Cox N R, Davis J K, Dybvig K, Cassell G H

Department of Microbiology, University of Alabama, Birmingham 35294.

Infect Immun. 1987 Nov;55(11):2838-40. doi: 10.1128/iai.55.11.2838-2840.1987.

The proteins of Mycoplasma pulmonis were examined by two-dimensional polyacrylamide gel electrophoresis and immunoblotting. Comparison of profiles from silver-stained two-dimensional polyacrylamide gel electrophoresis of 18 strains of M. pulmonis allowed identification of five proteins that were strain variable. These variable proteins were not dependent on the medium used to grow the organisms and were shown by reaction with serum samples from naturally infected rats and mice to be produced in vivo and not to be medium components. Identification of strain-variable proteins may lead to an explanation of the differences in properties found among M. pulmonis strains.

采用二维聚丙烯酰胺凝胶电泳和免疫印迹法对肺支原体的蛋白质进行了检测。对18株肺支原体银染二维聚丙烯酰胺凝胶电泳图谱的比较，确定了5种菌株可变蛋白。这些可变蛋白不依赖于用于培养微生物的培养基，通过与自然感染大鼠和小鼠的血清样本反应表明，它们是在体内产生的，而非培养基成分。菌株可变蛋白的鉴定可能有助于解释肺支原体菌株间特性差异的原因。