Department of Obstetrics and Gynecology, Shenzhen Baoan Women's and Children's Hospital, Jinan University, Shenzhen, 518102, Guangdong, China.
Department of Biobank, Shenzhen Baoan Women's and Children's Hospital, Jinan University, Shenzhen, 518102, Guangdong, China.
Sci Rep. 2020 Oct 29;10(1):18598. doi: 10.1038/s41598-020-75570-z.
The current biomarkers for postoperative fertility assessment caused by ovarian endometrioma (OE) are insufficient. The present study hypothesized that the peripheral lymphocyte subpopulation can be used as a candidate biomarker of postoperative infertility in OE. The association of the number of circulating CD4/CD8 T, NK, and γδ T cells with postoperative fertility was assessed in 33 OE patients aged 20 ~ 40 years between June 2018 and January 2019. Concomitantly, 68 healthy female subjects were recruited. The changes in the baseline immune characteristics between the two groups were compared. The data demonstrated significant differences in the ratio of CD4/CD8 T cells and the number of CD56+ NKG2D+ NK cells and γδ T cells between OE patients and control subjects. The patients were followed-up till December 2019 and the number of CD56+ NKG2D+ NK cells in the cases was a significant predictor for postoperative fertility as determined by different COX regression models (crude HR = 0.220, 95% CI = 0.059-0.822; adjusted HR = 0.127, 95% CI = 0.024-0.675). A significant delay to successful pregnancy was noted in OE patients (median time, 173 vs. 99 days, log-rank P = 0.013). The present findings suggested that CD56+ NKG2D+ NK cells are a candidate biomarker of postoperative fertility in OE patients. Larger population studies are warranted.
目前用于评估卵巢子宫内膜异位症（OE）术后生育能力的生物标志物还不够。本研究假设外周血淋巴细胞亚群可作为 OE 术后不孕的候选生物标志物。本研究评估了 20 至 40 岁的 33 例 OE 患者（2018 年 6 月至 2019 年 1 月）和 68 例健康女性的循环 CD4/CD8 T、NK 和 γδ T 细胞数量与术后生育能力的关系。比较两组之间基线免疫特征的变化。数据显示，OE 患者与对照组之间 CD4/CD8 T 细胞比值和 CD56+NKG2D+NK 细胞和 γδ T 细胞数量存在显著差异。对患者进行随访直至 2019 年 12 月，不同 COX 回归模型（未校正 HR=0.220，95%CI=0.059-0.822；校正 HR=0.127，95%CI=0.024-0.675）确定 CD56+NKG2D+NK 细胞数量是术后生育能力的显著预测因子。OE 患者成功妊娠的时间明显延迟（中位数时间，173 与 99 天，对数秩检验 P=0.013）。这些发现表明 CD56+NKG2D+NK 细胞是 OE 患者术后生育能力的候选生物标志物。需要更大的人群研究来验证。
