用于鉴定肠侵袭性大肠杆菌的DNA探针。
DNA probes for identification of enteroinvasive Escherichia coli.
作者信息
Gomes T A, Toledo M R, Trabulsi L R, Wood P K, Morris J G
机构信息
Department of Microbiology, Immunology, and Parasitology, Escola Paulista de Medicina, Sao Paulo, Brazil.
出版信息
J Clin Microbiol. 1987 Oct;25(10):2025-7. doi: 10.1128/jcm.25.10.2025-2027.1987.
Abstract
Eighty-one Escherichia coli strains belonging to all known invasive O serogroups were tested with two distinct invasiveness probes (pMR17 and pSF55). All 54 Sereny test-positive strains and 5 strains that lost Sereny positivity during storage hybridized with both probes. Probe-positive strains carried a 120- to 140-megadalton plasmid, did not produce lysine decarboxylase, and, with the exception of certain serotypes, were nonmotile. Motile strains of serotype O144:H25 were for the first time characterized as invasive by hybridization with the probes.
摘要
用两种不同的侵袭性探针（pMR17和pSF55）对81株属于所有已知侵袭性O血清群的大肠杆菌菌株进行了检测。所有54株塞雷尼试验阳性菌株以及5株在储存过程中失去塞雷尼阳性的菌株均与两种探针杂交。探针阳性菌株携带一个120至140兆道尔顿的质粒，不产生赖氨酸脱羧酶，并且除某些血清型外，无运动性。血清型为O144:H25的运动性菌株首次通过与探针杂交被鉴定为具有侵袭性。
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