• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

长链非编码RNA CCAL通过调控骨肉瘤中的miR-29b/ANGPTL4轴促进血管生成。

LncRNA CCAL Promotes Angiogenesis Through Regulating the MiR-29b/ANGPTL4 Axis in Osteosarcoma.

作者信息

Chen Shiyi, Yang Mingjia, Chang Shimin

机构信息

Department of Orthopaedic Surgery, Yangpu Hospital, Tongji University School of Medicine, Shanghai 200090, People's Republic of China.

Department of Epidemiology and Biostatistics, School of Public Health, Southeast University, Nanjing, Jiangsu Province 210096, People's Republic of China.

出版信息

Cancer Manag Res. 2020 Oct 23;12:10521-10530. doi: 10.2147/CMAR.S272230. eCollection 2020.

DOI:10.2147/CMAR.S272230
PMID:33122950
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7591080/
Abstract

PURPOSE

The objective of this study was to detect the expression of the long noncoding RNA (lncRNA) colorectal cancer-associated lncRNA (CCAL) in osteosarcoma tissues and to investigate its role in angiogenesis and the potential molecular mechanisms associated with this effect in osteosarcoma.

MATERIALS AND METHODS

CCAL expression in 40 osteosarcoma tissues and 40 noncancerous tissues was measured by qRT-PCR (quantitative real-time polymerase chain reaction). Tube formation assays were performed to explore the role of CCAL in angiogenesis in osteosarcoma. In addition, the regulatory interaction between CCAL, miR-29b, and ANGPTL4 was investigated via luciferase reporter assay and bioinformatics predictive analysis.

RESULTS

Compared with noncancerous tissues, the expression of CCAL was markedly upregulated in osteosarcoma tissues. Higher CCAL expression levels were closely related to shorter overall survival in patients with osteosarcoma. Additionally, functional analysis indicated that CCAL could facilitate tumour angiogenesis in vitro and in vivo in osteosarcoma. Mechanistically, CCAL upregulated ANGPTL4 expression in osteosarcoma cells, and ANGPTL4 mediated angiogenic induction by CCAL in osteosarcoma. Moreover, CCAL directly targeted miR-29b in osteosarcoma. More importantly, we demonstrated that CCAL upregulated the expression of ANGPTL4 by sponging miR-29b, which promoted angiogenesis in osteosarcoma.

CONCLUSION

Our results show that CCAL promotes angiogenesis by regulating the miR-29b/ANGPTL4 axis in osteosarcoma.

摘要

目的

本研究旨在检测长链非编码RNA（lncRNA）结直肠癌相关lncRNA（CCAL）在骨肉瘤组织中的表达，并探讨其在骨肉瘤血管生成中的作用以及与该效应相关的潜在分子机制。

材料与方法

采用qRT-PCR（定量实时聚合酶链反应）检测40例骨肉瘤组织和40例癌旁组织中CCAL的表达。进行管腔形成试验以探讨CCAL在骨肉瘤血管生成中的作用。此外，通过荧光素酶报告基因试验和生物信息学预测分析研究CCAL、miR-29b和ANGPTL4之间的调控相互作用。

结果

与癌旁组织相比，CCAL在骨肉瘤组织中的表达明显上调。CCAL表达水平较高与骨肉瘤患者较短的总生存期密切相关。此外，功能分析表明，CCAL在体外和体内均可促进骨肉瘤的肿瘤血管生成。机制上，CCAL上调骨肉瘤细胞中ANGPTL4的表达，且ANGPTL4介导CCAL在骨肉瘤中的血管生成诱导作用。此外，CCAL在骨肉瘤中直接靶向miR-29b。更重要的是，我们证明CCAL通过海绵化miR-29b上调ANGPTL4的表达，从而促进骨肉瘤中的血管生成。

结论

我们的结果表明，CCAL通过调节骨肉瘤中的miR-29b/ANGPTL4轴促进血管生成。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5666/7591080/ae124db435f7/CMAR-12-10521-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5666/7591080/589b077cf386/CMAR-12-10521-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5666/7591080/fa335a5dd8f5/CMAR-12-10521-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5666/7591080/8c21c1eab8fb/CMAR-12-10521-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5666/7591080/edb0fefd0681/CMAR-12-10521-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5666/7591080/ae124db435f7/CMAR-12-10521-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5666/7591080/589b077cf386/CMAR-12-10521-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5666/7591080/fa335a5dd8f5/CMAR-12-10521-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5666/7591080/8c21c1eab8fb/CMAR-12-10521-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5666/7591080/edb0fefd0681/CMAR-12-10521-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5666/7591080/ae124db435f7/CMAR-12-10521-g0005.jpg

相似文献

1
LncRNA CCAL Promotes Angiogenesis Through Regulating the MiR-29b/ANGPTL4 Axis in Osteosarcoma.长链非编码RNA CCAL通过调控骨肉瘤中的miR-29b/ANGPTL4轴促进血管生成。
Cancer Manag Res. 2020 Oct 23;12:10521-10530. doi: 10.2147/CMAR.S272230. eCollection 2020.
2
Up-regulation of long noncoding RNA CCAL predicts poor patient prognosis and promotes tumor metastasis in osteosarcoma.长链非编码RNA CCAL的上调预示骨肉瘤患者预后不良并促进肿瘤转移。
Int J Biol Markers. 2017 Mar 2;32(1):e108-e112. doi: 10.5301/jbm.5000240.
3
Long non-coding RNA CCAL/miR-149/FOXM1 axis promotes metastasis in gastric cancer.长非编码 RNA CCAL/miR-149/FOXM1 轴促进胃癌转移。
Cell Death Dis. 2018 Sep 24;9(10):993. doi: 10.1038/s41419-018-0969-z.
4
The role and clinical significance of long noncoding RNA zinc finger E-box-binding homeobox two antisense RNA 1 in promoting osteosarcoma cancer cell proliferation, inhibiting apoptosis and increasing migration by regulating miR-145.长非编码 RNA 锌指 E 盒结合同源盒 2 反义 RNA 1 通过调控 miR-145 促进骨肉瘤癌细胞增殖、抑制凋亡和增加迁移的作用及临床意义。
Anticancer Drugs. 2021 Feb 1;32(2):168-177. doi: 10.1097/CAD.0000000000000984.
5
Long noncoding RNA CCAL promotes gastric cancer cell proliferation and migration in a Myc dependent way.长链非编码RNA CCAL以Myc依赖的方式促进胃癌细胞增殖和迁移。
Pharmazie. 2018 Jan 2;73(1):42-48. doi: 10.1691/ph.2018.7721.
6
Circular RNA COL1A2 promotes angiogenesis via regulating miR-29b/VEGF axis in diabetic retinopathy.环状 RNA COL1A2 通过调控 miR-29b/VEGF 轴促进糖尿病视网膜病变中的血管生成。
Life Sci. 2020 Sep 1;256:117888. doi: 10.1016/j.lfs.2020.117888. Epub 2020 Jun 1.
7
Long noncoding RNA PVT1 promotes metastasis via miR-484 sponging in osteosarcoma cells.长链非编码 RNA PVT1 通过海绵吸附 miR-484 促进骨肉瘤细胞转移。
Eur Rev Med Pharmacol Sci. 2020 Mar;24(5):2229-2238. doi: 10.26355/eurrev_202003_20488.
8
Long noncoding RNA CRNDE functions as a diagnostic and prognostic biomarker in osteosarcoma, as well as promotes its progression via inhibition of miR-335-3p.长链非编码 RNA CRNDE 作为骨肉瘤的诊断和预后生物标志物发挥作用，并且通过抑制 miR-335-3p 促进其进展。
J Biochem Mol Toxicol. 2021 May;35(5):e22734. doi: 10.1002/jbt.22734. Epub 2021 Feb 1.
9
LncRNA GSEC promotes the proliferation, migration and invasion by sponging miR-588/ EIF5A2 axis in osteosarcoma.长链非编码 RNA GSEC 通过海绵吸附 miR-588/EIF5A2 轴促进骨肉瘤的增殖、迁移和侵袭。
Biochem Biophys Res Commun. 2020 Nov 5;532(2):300-307. doi: 10.1016/j.bbrc.2020.08.056. Epub 2020 Aug 29.
10
Long non-coding RNA CCAL promotes hepatocellular carcinoma progression by regulating AP-2α and Wnt/β-catenin pathway.长链非编码 RNA CCAL 通过调控 AP-2α 和 Wnt/β-连环蛋白通路促进肝细胞癌进展。
Int J Biol Macromol. 2018 Apr 1;109:424-434. doi: 10.1016/j.ijbiomac.2017.12.110. Epub 2017 Dec 22.

引用本文的文献

1
Emerging roles of angiopoietin‑like 4 in human tumors (Review).血管生成素样4在人类肿瘤中的新作用（综述）
Int J Oncol. 2025 Feb;66(2). doi: 10.3892/ijo.2024.5715. Epub 2024 Dec 20.
2
Osteosarcoma in a ceRNET perspective.中枢神经系统外尤文肉瘤的临床特征。
J Biomed Sci. 2024 Jun 5;31(1):59. doi: 10.1186/s12929-024-01049-y.
3
Tumor-Derived Exosomal miR-29b Reduces Angiogenesis in Pancreatic Cancer by Silencing ROBO1 and SRGAP2.肿瘤衍生的外泌体 miR-29b 通过沉默 ROBO1 和 SRGAP2 减少胰腺癌中的血管生成。

本文引用的文献

1
Prognostic Significance of CD105- and CD31-Assessed Microvessel Density in Paired Biopsies and Surgical Samples of Laryngeal Carcinoma.CD105和CD31评估的微血管密度在喉癌配对活检及手术样本中的预后意义
Cancers (Basel). 2020 Jul 25;12(8):2059. doi: 10.3390/cancers12082059.
2
LncRNA DANCR promotes tumor growth and angiogenesis in ovarian cancer through direct targeting of miR-145.长链非编码 RNA DANCR 通过靶向 miR-145 促进卵巢癌的肿瘤生长和血管生成。
Mol Carcinog. 2019 Dec;58(12):2286-2296. doi: 10.1002/mc.23117. Epub 2019 Sep 23.
3
Long noncoding RNA CCAL transferred from fibroblasts by exosomes promotes chemoresistance of colorectal cancer cells.
J Immunol Res. 2022 Oct 14;2022:4769385. doi: 10.1155/2022/4769385. eCollection 2022.
4
Oncogenic Role of Exosomal Circular and Long Noncoding RNAs in Gastrointestinal Cancers.外泌体环状和长非编码 RNA 在胃肠道癌症中的致癌作用。
Int J Mol Sci. 2022 Jan 15;23(2):930. doi: 10.3390/ijms23020930.
5
LncRNA MALAT1 Promotes Tumor Angiogenesis by Regulating MicroRNA-150-5p/VEGFA Signaling in Osteosarcoma: and Analyses.长链非编码RNA MALAT1通过调控骨肉瘤中的微小RNA-150-5p/血管内皮生长因子A信号促进肿瘤血管生成： 及分析
Front Oncol. 2021 Oct 7;11:742789. doi: 10.3389/fonc.2021.742789. eCollection 2021.
6
A New Light on Potential Therapeutic Targets for Colorectal Cancer Treatment.结直肠癌治疗潜在治疗靶点的新见解
Biomedicines. 2021 Oct 10;9(10):1438. doi: 10.3390/biomedicines9101438.
长链非编码 RNA CCAL 通过外泌体从成纤维细胞转移促进结直肠癌细胞的化疗耐药性。
Int J Cancer. 2020 Mar 15;146(6):1700-1716. doi: 10.1002/ijc.32608. Epub 2019 Aug 27.
4
Long non-coding RNA Taurine upregulated gene 1 promotes osteosarcoma cell metastasis by mediating HIF-1α via miR-143-5p.长链非编码 RNA 牛磺酸上调基因 1 通过 miR-143-5p 介导 HIF-1α 促进骨肉瘤细胞转移。
Cell Death Dis. 2019 Mar 25;10(4):280. doi: 10.1038/s41419-019-1509-1.
5
Obesity-associated inflammation promotes angiogenesis and breast cancer via angiopoietin-like 4.肥胖相关炎症通过血管生成素样蛋白 4 促进血管生成和乳腺癌。
Oncogene. 2019 Mar;38(13):2351-2363. doi: 10.1038/s41388-018-0592-6. Epub 2018 Dec 5.
6
Long non-coding RNA CCAL/miR-149/FOXM1 axis promotes metastasis in gastric cancer.长非编码 RNA CCAL/miR-149/FOXM1 轴促进胃癌转移。
Cell Death Dis. 2018 Sep 24;9(10):993. doi: 10.1038/s41419-018-0969-z.
7
Angiopoietin-like 4 promotes osteosarcoma cell proliferation and migration and stimulates osteoclastogenesis.血管生成素样蛋白 4 促进骨肉瘤细胞增殖和迁移，并刺激破骨细胞生成。
BMC Cancer. 2018 May 8;18(1):536. doi: 10.1186/s12885-018-4468-5.
8
Noncoding RNA:RNA Regulatory Networks in Cancer.非编码 RNA：癌症中的 RNA 调控网络。
Int J Mol Sci. 2018 Apr 27;19(5):1310. doi: 10.3390/ijms19051310.
9
Long Noncoding RNA CCAL Promotes Papillary Thyroid Cancer Progression by Activation of NOTCH1 Pathway.长链非编码RNA CCAL通过激活NOTCH1信号通路促进甲状腺乳头状癌进展。
Oncol Res. 2018 Oct 17;26(9):1383-1390. doi: 10.3727/096504018X15188340975709. Epub 2018 Feb 22.
10
Long noncoding RNAs in cancer cells.癌细胞中的长非编码 RNA
Cancer Lett. 2018 Apr 10;419:152-166. doi: 10.1016/j.canlet.2018.01.053.