Marra Valentina, Abballe Annalisa, Dellatte Elena, Iacovella Nicola, Ingelido Anna Maria, De Felip Elena
Istituto Superiore di Sanità, Dipartimento Ambiente e Salute, Viale Regina Elena 299, Roma 00161, Italy.
Int J Anal Chem. 2020 Oct 16;2020:8878618. doi: 10.1155/2020/8878618. eCollection 2020.
Per- and polyfluorinated alkyl substances (PFASs) are ubiquitous environmental contaminants, present in the environment and in the human body. They have raised global concern because of their diffusion in the environment, particularly in water, causing cases of human overexposure due to consumption of contaminated drinking water. Human biomonitoring is the most effective way to characterize human exposure to PFASs, and it is important that as many labs as possible could easily perform this kind of analysis. Analytical methods for quantitation of PFAS mixtures in human serum have been developed, but most of them required materials that are not always easily available in all the laboratories. This paper describes a very simple and accessible HPLC MS/MS method of analysis and quantification of 13 perfluorocarboxylic acids and perfluorosulfonate compounds (belonging to the class of per- and polyfluorinated alkyl substances (PFASs)) in human serum. Method development data provide detailed descriptions of the optimization process in terms of sample preparation, laboratory analysis of human serum samples, determination of analytes by HPLC MS/MS, and describing the pump gradient time, working conditions, and acquisition.
全氟和多氟烷基物质（PFASs）是普遍存在的环境污染物，存在于环境和人体中。由于它们在环境中扩散，特别是在水中扩散，导致因饮用受污染的饮用水而出现人体过度暴露的情况，因此引起了全球关注。人体生物监测是表征人体接触PFASs的最有效方法，并且尽可能多的实验室能够轻松进行此类分析非常重要。已经开发了用于定量人血清中PFAS混合物的分析方法，但其中大多数方法所需的材料并非在所有实验室都容易获得。本文描述了一种非常简单且易于操作的HPLC MS/MS方法，用于分析和定量人血清中的13种全氟羧酸和全氟磺酸化合物（属于全氟和多氟烷基物质（PFASs）类别）。方法开发数据提供了在样品制备、人血清样品的实验室分析、通过HPLC MS/MS测定分析物以及描述泵梯度时间、工作条件和采集方面优化过程的详细描述。
